Smo (BC048091) Mouse Untagged Clone

CAT#: MC218049

Smo (untagged) - Mouse smoothened homolog (Drosophila) (cDNA clone MGC:57078 IMAGE:6486595), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Smo
• Synonyms: D13Mgi8, Smoh
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC048091
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCTGGCCGCCCCGTGCGTGGGCCCGAGCTGGCGCCCCGGAGGCTGCTGCAGTTGCTGCTGCTGG
  TACTGCTGGGGGGCCCGGGCCGGGGGGCGGCCTTGAGCGGGAACGTGACCGGGCCTGGGCCTCACAGCGC
  CAGCGGGAGCTCGAGGAGGAACGTGCCGGTGACCAGCCCTCCGCCGCCGCTGCTGAGCCACTGCGGCCGG
  GCCGCCCACTGCGAGCCTTTGCGCTACAACGTGTGCCTGGGCTCGGCGCTGCCCTACGGAGCCACCACCA
  CGCTGCTGGCTGGGGACTCGGACTCGCAGGAGGAAGCGCACGGCAAGCTCGTGCTCTGGTCCGGCCTCCG
  GAATGCCCCCCGCTGCTGGGCAGTGATCCAGCCCCTGCTGTGTGCTGTCTACATGCCCAAGTGTGAGAAT
  GACCGAGTGGAGTTGCCCAGCCGTACCCTCTGCCAGGCCACCCGAGGCCCCTGTGCCATTGTGGAGCGGG
  AGCGAGGGTGGCCTGACTTTCTGCGTTGCACACCGGACCACTTCCCTGAAGGCTGCCCAAACGAGGTACA
  AAACATCAAGTTCAACAGCTCAGGCCAATGTGAAGCACCCTTGGTGCGAACAGACAACCCCAAGAGCTGG
  TATGAGGACGTGGAGGGCTGTGGGATTCAGTGTCAGAACCCGCTGTTCACCGAGGCCGAGCACCAGGACA
  TGCACAGCTACATCGCAGCCTTCGGGGCGGTCACTGGTCTCTGCACGCTCTTCACACTGGCCACCTTTGT
  GGCTGACTGGCGGAACTCCAATCGCTACCCTGCGGTTATTCTCTTCTATGTCAATGCGTGTTTCTTCGTG
  GGCAGCATTGGCTGGCTGGCCCAGTTCATGGATGGTGCCCGCCGAGAGATTGTTTGCCGAGCAGATGGCA
  CCATGAGATTTGGGGAGCCCACCTCCAGTGAGACCCTGTCCTGTGTCATCATCTTTGTCATTGTGTACTA
  TGCCTTGATGGCTGGAGTAGTCTGGTTCGTGGTCCTCACCTATGCCTGGCACACCTCCTTCAAAGCCCTG
  GGCACCACCTACCAGCCTCTCTCGGGCAAGACATCCTATTTCCACCTGCTCACGTGGTCACTCCCCTTTG
  TCCTCACGGTGGCAATCCTGGCTGTGGCTCAGGTAGATGGAGACTCCGTGAGTGGCATCTGTTTTGTAGG
  CTACAAGAACTATCGGTACCGTGCTGGCTTTGTCCTGGCCCCAATTGGCCTGGTGCTTATTGTGGGAGGC
  TACTTCCTCATCACAGGGGTCATGACTCTGTTCTCCATCAAGAGCAACCACCCCTGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC048091
• Insert Size: 1320 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC048091, AAH48091
• RefSeq Size: 1747 bp
• RefSeq ORF: 1319 bp
• Locus ID: 319757
• Cytogenetics: 6 12.36 cM
• Gene Summary: G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO) (By similarity). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia. Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (PubMed:25644602).[UniProtKB/Swiss-Prot Function]