Dusp7 (BC010207) Mouse Untagged Clone

CAT#: MC218030

Dusp7 (untagged) - Mouse dual specificity phosphatase 7 (cDNA clone MGC:11703 IMAGE:3964527), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dusp7
• Synonyms: MKPX, MKP-X, PYST2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC010207
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGCCGCCTGCGCAAAGGCAACCTGCCCATCCGCTCCATCATTCCCAACCATGCCGACAAGGAGC
  GCTTCGCCACGCGCTGCAAGGCGGCCACCGTGCTGCTCTACGACGAGGCCACGGCGGAGTGGCAGCCCGA
  GCCCGGCGCCCCCGCCTCCGTTCTCGGCCTGCTCCTGCAGAAGCTGCGAGACGACGGCTGCCAGGCCTAC
  TACCTCCAAGGTGGTTTCAACAAGTTCCAGACGGAATACTCTGAGCATTGTGAGACTAACGTGGACAGCT
  CGTCCTCCCCGAGTGGCTCGCCACCCACCTCCGTGCTGGGCCTGGGGGGCCTGCGCATCAGCTCTGACTG
  CTCAGACGGCGAGTCGGACCGAGAGCTGCCCAGCAGTGCCACTGAGTCGGATGGCAGCCCTGTACCATCC
  AGCCAGCCGGCCTTTCCGGTCCAGATCCTGCCCTACCTCTACCTCGGCTGTGCCAAGGACTCTACCAACC
  TGGATGTACTTGGCAAATACGGCATCAAATACATCCTCAACGTCACACCCAACCTGCCCAACGCCTTTGA
  GCACGGTGGTGAGTTCACCTACAAGCAAATCCCCATCTCTGACCACTGGAGCCAGAACCTCTCTCAGTTC
  TTCCCCGAGGCCATCAGCTTCATTGATGAAGCCCGCTCCAAGAAGTGTGGTGTCTTGGTGCACTGCCTAG
  CAGGCATCAGCCGTTCAGTGACAGTTACTGTAGCCTACCTGATGCAGAAGATGAACCTGTCCCTCAATGA
  TGCCTATGACTTCGTCAAGAGGAAAAAGTCTAACATCTCGCCCAACTTCAACTTCATGGGGCAGCTGCTG
  GACTTTGAGCGGACGCTGGGGCTCAGCAGCCCATGCGACAACCACGCACCCAGTGAGCAGCTCTACTTCT
  CCACGCCCACCAACCATAACCTGTTCCCGATCAACACGCTCGAGTCCACGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC010207
• Insert Size: 963 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC010207, AAH10207
• RefSeq Size: 2584 bp
• RefSeq ORF: 962 bp
• Locus ID: 235584
• Cytogenetics: 9 F1
• Gene Summary: Dual specificity protein phosphatase (PubMed:27783954). Shows high activity towards MAPK1/ERK2 (By similarity). Also has lower activity towards MAPK14 and MAPK8 (By similarity). In arrested oocytes, plays a role in meiotic resumption (PubMed:27783954). Promotes nuclear envelope breakdown and activation of the CDK1/Cyclin-B complex in oocytes, probably by dephosphorylating and inactivating the conventional protein kinase C (cPKC) isozyme PRKCB (PubMed:27783954). May also inactivate PRKCA and/or PRKCG (PubMed:27783954). Also important in oocytes for normal chromosome alignment on the metaphase plate and progression to anaphase, where it might regulate activity of the spindle-assembly checkpoint (SAC) complex (PubMed:27783954).[UniProtKB/Swiss-Prot Function]