Ddx27 (BC011321) Mouse Untagged Clone

CAT#: MC218009

Ddx27 (untagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (cDNA clone MGC:18747 IMAGE:4007058), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ddx27
• Synonyms: C86129
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC011321
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCTCTCCCGGCCCCTCCTGAAGGCCATTACAGCCATGGGCTTCAAGCAGCCCACGCCAATCCAGA
  AGGCGTGTATCCCTGTGGGTCTGCTGGGGAAGGACATCTGCGCCTGTGCAGCCACTGGGACAGGCAAAAC
  TGCAGCCTTTGCACTGCCCGTCTTGGAGCGTCTGATCTACAAGCCCCGCCAGGCTGCAGTCACTCGCGTG
  CTGGTGCTGGTCCCCACGAGAGAGCTGGGCATCCAAGTGCACTCTGTCACCAAGCAGCTGGCCCAGTTCT
  GCAGCATCACTACCTGCCTGGCTGTGGGTGGCCTGGATGTGAAGTCTCAGGAAGCCGCTCTTCGGGCAGC
  ACCAGACATCCTCATTGCCACACCTGGCCGGCTCATCGACCACCTCCATAACTGCCCCTCCTTCCACCTG
  AGCAGCATTGAAGTGCTCATCTTGGATGAGGCTGACAAGATGCTGGATGAGTATTTCGAGGAGCAGATGA
  AGGAGATTATCCGAATGTGTTCCCACCACCGCCAGACCATGCTCTTCTCGGCCACCATGACAGATGAGGT
  GAAAGATCTGGCTTCGGTCTCCTTAAAGAATCCTGTGCGGATATTTGTGAACAGCAACACAGATGTCGCT
  CCCTTCCTGCGGCAAGAGTTCATACGGATCCGGCCTAATAGGGAAGGGGACCGGGAGGCCATTGTGGCAG
  CTCTGCTGATGAGAACCTTCACTGACCACGTGATGCTCTTCACCCAGACCAAGAAGCAGGCCCATCGCAT
  GCACATCCTCCTGGGGCTGCTGGGGCTGCAGGTGGGCGAGCTCCACGGCAACTTGTCACAGACCCAGCGC
  CTGGAGGCCCTCCGGCGCTTTAAGGACGAACAGATTGACATCCTGGTGGCCACAGATGTGGCAGCCCGCG
  GTCTTGACATTGAAGGAGTGAAAACAGTGATCAACTTCACCATGCCCAACACTGTTAAACACTATGTGCA
  CCGGGTGGGGCGGACGGCCCGTGCTGGCAGGGCTGGACGCTCTGTCTCCCTAGTGGGAGAGGAGGAGCGG
  AAGATGCTGAAGGAGATCGTGAAGGCTGCCAAGGCGCCAGTGAAGGCTCGGATCCTTCCCCAGGATGTTA
  TCCTCAAGTTCCGGGACAAGATTGAGAAATTGGAGAAAGATGTGTATGCAGTGCTGCAGCTGGAGGCGGA
  GGAAAAAGAGATGCAGCAGTCGGAAGCCCAGATCGACACAGCACAGCGACTCTTAGCCAAAGGGAAAGAG
  ACAGCAGACCAGGAGCCTGAGAGGAGCTGGTTCCAGACCAAAGAAGAGAGGAAGAAGGAGAAGATTGCTA
  AGGCTCTACAGGAGTTTGACTTGGCCTTAAGAGGAAAGAAGAAAAGGAAGAAGTTTATGAAGGATGCCAA
  GAAAAAGGGGGAGATGACGGCAGAGGAGAGGTCCCAGTTTGAAATCCTCAAGGCACAGATGTTTGCAGAG
  CGGCTGGCCAAGAGGAATCGCAGAACCAAGCGAGCACGGGCAATGCCTGAGGACGAGCCTACAGGCCCTG
  CTAAAAAGCAGAAGCAGCAGCAGAAGTCTGTGTTTGATGAAGAACTCACCAACACAAGCAAGAAGGCCCT
  GAAACAGTATCGGGCTGGCCCTTCCTTTGAAGAGAGGAAACAGTCAGGTCTGCCTCGCCAGAGGAGAGGG
  AACTTCAAATCTAAGTCCAGGTACAAGAGAAAGAAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC011321
• Insert Size: 1719 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC011321, AAH11321
• RefSeq Size: 2483 bp
• RefSeq ORF: 1718 bp
• Locus ID: 228889
• Cytogenetics: 2 H3
• Gene Summary: Probable ATP-dependent RNA helicase. Component of the nucleolar ribosomal RNA (rRNA) processing machinery that regulates 3' end formation of ribosomal 47S rRNA.[UniProtKB/Swiss-Prot Function]