Pfkfb3 (NM_001177757) Mouse Untagged Clone

CAT#: MC217960

Pfkfb3 (untagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), transcript variant 7, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pfkfb3
• Synonyms: E330010H22Rik; iPFK-2; uPFK-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217960 representing NM_001177757
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGTTGGAACTGACCCAGAGCCGGGTACAGAAGATCTGGGTGCCCGTCGATCACCGACCCTCGTTGC
  CCAGATCCTGTGGGCCGAAGCTGACCAACTCCCCAACCGTGATTGTGATGGTGGGGCTCCCAGCCCGGGG
  TAAGACTTACATCTCCAAGAAGCTGACTCGCTACCTCAACTGGATAGGTGTTCCAACGAAAGTGTTCAAT
  GTGGGAGAGTATCGGCGTGAGGCTGTGAAGCAGTACAGCTCTTACAACTTCTTCCGCCCTGACAATGAGG
  AAGCCATGAGAGTCCGAAAACAGTGTGCCTTAGCTGCCCTGAGAGATGTCAAAAGCTACCTGACAAAGGA
  AGGTGGACAGATTGCAGTTTTTGATGCCACCAATACAACTAGAGAAAGAAGGCACATGATCCTTAATTTT
  GCCAAAGAAAATGACTTCAAGGCATTTTTCATCGAGTCGGTCTGTGACGACCCTACTGTTGTGGCCTCCA
  ACATCATGGAAGTTAAAATCTCCAGCCCGGATTACAAAGACTGCAACTCAGCAGAAGCCATGGACGACTT
  CATGAAGAGAATCAATTGCTATGAAGCCAGCTACCAGCCTCTTGACCCTGATAAATGTGACAGGGACTTG
  TCACTCATCAAAGTGATTGATGTTGGCCGGAGGTTCTTGGTGAACAGAGTGCAGGACCACATCCAGAGCC
  GAATTGTATACTACCTGATGAACATCCACGTGCAGCCCCGTACCATCTACCTGTGTCGGCATGGCGAGAA
  TGAGTACAACTTGCAGGGCAAGATTGGGGGCGACTCAGGCTTGTCCAGCAGAGGCAAGAAGTTCGCCAAT
  GCCCTGAGCAAGTTCGTAGAAGAACAGAATCTGAAGGACCTCCGTGTGTGGACCAGCCAGCTGAAAAGTA
  CCATTCAGACCGCGGAGGCCCTGAGGCTCCCCTACGAGCAGTGGAAGGCACTCAACGAGATCGATGCTGG
  TGTGTGTGAGGAACTAACCTATGAGGAGATCAGAGATACCTACCCTGAGGAGTACGCACTGCGTGAACAG
  GACAAGTACTACTATCGCTATCCCACGGGAGAGTCCTACCAGGATCTTGTCCAACGCCTGGAGCCTGTGA
  TCATGGAATTAGAGCGCCAAGAGAATGTATTGGTCATCTGTCACCAGGCTGTTCTACGCTGCCTACTAGC
  CTACTTCCTCGACAAGAGTGCAGAGGAGATGCCATACCTGAAATGTCCGCTCCACACTGTCCTGAAACTG
  ACACCTGTGGCATATGGGTGCCGGGTGGAGTCCATTTACTTGAATGTAGAATCGGTGAGCACACACCGGG
  AGAGGTCAGAGGATGCAAAGAAGGGACCTAACCCGCTCATGAGACGCAATAGTGTCACCCCACTAGCCAG
  TCCCGAGCCCACCAAAAAGCCTCGCATCAACAGCTTTGAGGAGCGTGTGGCTTCCACTTCTGCTGCCCTG
  CCAAGCTGCCTGCCCCCGGAAGTGCCCACGCAGCTGCCCGGACAACCTTTGCTAGGGAAAGCCTGTCTAC
  GAAGTGTCTGTCACATTTTTTCAAAGTTTTCTCCTTACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001177757
• Insert Size: 1581 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177757.1, NP_001171228.1
• RefSeq Size: 5119 bp
• RefSeq ORF: 1581 bp
• Locus ID: 170768
• Cytogenetics: 2 A1