4930579E17Rik (BC096030) Mouse Untagged Clone

CAT#: MC217914

4930579E17Rik (untagged) - Mouse RIKEN cDNA 4930579E17 gene (cDNA clone MGC:106594 IMAGE:30610161), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: 4930579E17Rik
• Synonyms: 4930579E17Rik; AV040780
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC096030
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCTGGGCCGTGCAGCAGGCCCGCTGAGCCTGGGCATTGCGTGAGCGGCCCCGCGGGCGCGGGCT
  CAGCGTTCCCGGAGTCCCCGTTGTCCGTCGCTGGGGCGGAGCCCGGGAACCGCCCTGGAACCGTGGCCGC
  CGTGCTGCCGGCTGGGGGTTGCGGGGAAAGGATGGGCGTCCGCACCCCGAAGCAGTTCTGCCGGGTTCTG
  GAAAGGCCGCTCATCAGCTACACTTTGCAGGCTATGGAGAGAGTATGCTGGATAAAGGACATTGTTGTGA
  CAGTGACAGGGGAGAACATGGAAGCAATGAGAAGTATCATCCAGAGGTATGGGCATAAGCGCATCTCACT
  AGCTGAGGCTGGAGCCACGCGCCACAGATCAATTTTCAATGGACTGAAAGCCCTGGCAGAAGATCAGCCA
  GACTGTAAACTCACTAAGCCAGAAGTGGTGATTATCCATGACGCCGTGAGACCTTTTGTTGAGGAAGATA
  TCCTCCTGAGAGTTGTCTTAGCAGCTAAGGAACATGGGGCAGCAGGAGCAATTCGACCTCTGGTGTCCAC
  TGTCATCAGTCCCTCTGCTGATGGTCACTTAGACCACTCACTGGACCGTGCCAAGCATAGGGCAAGCGAA
  ATGCCCCAGGCTTTTCTCTTTGATGTCATCTATGAAGCGTATCAGCAGTGTAGTGATTTTGACTTGGAAT
  TTGGAACAGAGTGCTTGCAGTTGGCTCTAAAATACTGTCACAGGAAAGCAAAACTTGTAGAAGGGCCCCC
  TGCCCTCTGGAAGGTGACCTACAAACAAGACCTGTGTGCAGCTGAAGCCATGATTAAAGGTGTGTTCAAC
  CTTGTGACTGTCAGCGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC096030
• Insert Size: 861 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC096030, AAH96030
• RefSeq Size: 3142 bp
• RefSeq ORF: 860 bp
• Locus ID: 75847
• Cytogenetics: 12 A3
• Gene Summary: Cytidylyltransferase required for protein O-linked mannosylation (By similarity). Catalyzes the formation of CDP-ribitol nucleotide sugar from D-ribitol 5-phosphate (By similarity). CDP-ribitol is a substrate of FKTN during the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (By similarity). Shows activity toward other pentose phosphate sugars and mediates formation of CDP-ribulose or CDP-ribose using CTP and ribulose-5-phosphate or ribose-5-phosphate, respectively (By similarity). Not Involved in dolichol production (By similarity).[UniProtKB/Swiss-Prot Function]