Ranbp10 (BC024698) Mouse Untagged Clone

CAT#: MC217908

Ranbp10 (untagged) - Mouse RAN binding protein 10 (cDNA clone MGC:30446 IMAGE:3667000), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ranbp10
• Synonyms: 4432417N03Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC024698
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAATTGGACTCTCAGCGCAAGGCGTTAACATGAACAGACTGCCTGGTTGGGACAAGCATTCCTATG
  GTTACCATGGCGATGATGGGCATTCCTTCTGCTCCTCTGGGACAGGCCAGCCTTATGGTCCCACATTCAC
  CACAGGGGATGTGATCGGCTGTTGTGTCAACCTCATCAATGGCACCTGCTTCTACACCAAGAATGGCCAC
  AGCCTTGGTATCGCCTTCACAGATCTCCCGGCCAACCTCTACCCCACTGTCGGCCTACAGACACCTGGGG
  AGATTGTGGATGCCAACTTTGGGCAGCAGCCCTTCCTGTTCGACATTGAGGACTATATGCGGGAGTGGCG
  TGCCAAAGTCCAGGGCACTGTGCACGGCTTTCCCATAAGTGCCCGGCTTGGCGAGTGGCAGGCGGTGCTG
  CAGAACATGGTCTCGTCTTACCTGGTGCATCATGGGTATTGTTCCACAGCCACAGCTTTTGCCCGAATGA
  CTGAAACCCCGATTCAGGAAGAGCAGGCATCCATAAAGAACAGACAAAAAATCCAGAAGCTGGTGCTGGA
  AGGCCGGGTGGGTGAAGCCATTGAGACAACCCAGCGCTTCTACCCTGGGCTGCTGGAACATAATCCCAAC
  CTGCTCTTCATGCTCAAGTGTCGACAGTTCGTGGAGATGGTGAACGGCACTGACAGTGAGGTCCGCAGCC
  TGAGTTCCCGAAGCCCCAAGTCCCAGGACAGCTACCCTGGCTCCCCCAGCCTCAGCCCCCGACATGGCCC
  CAGTAGTTCCCATATACACAACACAGGAGCAGACAGTCCCAGCTGCAGCAATGGCGTCGCATCCACCAAG
  AACAAACAGAACCACAGTAAATACCCCGCACCCAGCTCCTCCTCCTCGTCCTCATCCTCCTCGTCCTCCT
  CTTCCCCGTCCTCCGTCAATTACTCCGAGTCCAACTCAACAGACTCTACCAAGTCCCAGCCCCACAGCAG
  TACCAGTAACCAGGAGACCAGTGATAGCGAGATGGAGATGGAGGCAGAGCACTATCCCAATGGTGTGCTG
  GAAAGCGTCTCCACGCGCATCGTCAATGGGGCCTATAAGCATGACGACCTGCAGACAGATGAGTCCAGCA
  TGGACGACGGGCATCCACGGAGGCAGCTCTGCGGGGGCAACCAGGCTGCCACAGAAAGGATCATCCTGTT
  TGGCCGAGAGCTGCAGGCATTGAGCGAGCAGCTGGGCCGGGAGTACGGCAAGAACTTGGCCCACACGGAG
  ATGCTGCAGGATGCCTTTAGCCTACTAGCATACTCAGACCCCTGGAGCTGCCCAGTAGGTCATCAGCTTG
  ATCCCATCCAGAGAGAGCCCGTGTGTGCTGCTCTCAACAGCGCCATTTTAGAGTCTCAGAACCTGCCAAA
  GCAGCCTCCTTTGATGCTCGCCCTGGGCCAGGCATCTGAATGTCTACGGCTCATGGCCCGAGCAGGCCTG
  GGATCTTGCTCCTTTGCCAGAGTCGATGACTACTTGCACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC024698
• Insert Size: 1512 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC024698, AAH24698
• RefSeq Size: 5169 bp
• RefSeq ORF: 1511 bp
• Locus ID: 74334
• Cytogenetics: 8 D3
• Gene Summary: May act as an adapter protein to couple membrane receptors to intracellular signaling pathways (Probable). Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1 (By similarity). Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation (By similarity). Acts as a guanine nucleotide exchange factor (GEF) for RAN GTPase (PubMed:18347012). May play an essential role in hemostasis and in maintaining microtubule dynamics with respect to both platelet shape and function (PubMed:19801445).[UniProtKB/Swiss-Prot Function]