Dus1l (BC019480) Mouse Untagged Clone

CAT#: MC217857

Dus1l (untagged) - Mouse dihydrouridine synthase 1-like (S. cerevisiae) (cDNA clone MGC:28421 IMAGE:4037583), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dus1l
• Synonyms: 1110032N12Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC019480
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATAGCCAAGAGAGGTCACTATGGTGCTTTTCTGCAGGAGGAGTGGGACCTTCTTCAAAGAATGATTC
  TGTTGGCTCATGAGCGACTCTCTGTTCCTGTCACGTGCAAAATTCGTGTCTTCCCAGAAATTGACAAGAC
  AGTGAGGTACGCCCAGATGCTGGAGAAGGCTGGCTGTCAGCTGCTGACTGTGCATGGGCGCACCAAGGAG
  CAGAAGGGGCCCATGGCAGGAACAGCCTCCTGGGAACACATCAAGGCCGTTCGGAAGGCTGTGGGAATCC
  CTGTGTTTGCCAATGGGAACATCCAATGCCTGCAGGATGTGGAGCGGTGCATCCAGGACACCGGCGTGCA
  GGGAGTCATGAGTGCAGAGGGGAACCTGCACAACCCTGCCCTCTTTGAGGGCCGGAGTCCTGCTGTGTGG
  GAGCTGGCCGAGGAGTACCTGGACATTGTGCGGCAACACCCTTGTCCACTATCGTATGTCCGGGCCCATC
  TCTTCAAACTGTGGCACCACACGCTACAGGTACATCAGCAACTTCGAGAAGAGCTGGCCAAAGTGAAGAC
  CCTGGAGGGCGTGGCTGCTGTGAGCCAGGCGCTAAAGCTTCGGTGTCAGGAGGACATGTCTAGGCAGCAA
  GAAGGAGTGAGGCCAGCTGACAACTTACCTGCTTTCCATTGGATCTGCCAGCCGTACATCCGGCCAGGAC
  CTAGGGAAGGGAGCAAGGAGAATAGTGGTGGTCGAAGCAAGCGGGCTCTGGAGGAAGAAGAAGGCAGCAT
  GGAGGGCTTGTCCAAGAATAAGCTGAAGAAACAACTGAGGAACCCTCACAAAACCTTTGACCCTTCCCTG
  AAACCCAAATATGCCAAGTGTGACCAGTGTGGAAATCCAAAGGGCAATAGGTGTGTGTTTAATCTGTGCC
  GTGGCTGCTGCAAGAAGCGAGCTTTCAGAGAGACAGCAGATTGCCCAGGTCATGGATTGCTTTTTAAGAC
  CAAATTAGAGAAGTCTTTGGCCTGGAAAGGGACCCAGCCTGGACTGCAGGAAGCCCAGCAGGTGAGGCCT
  GTGACACCAAGTGGTTTCTCTGAAGTCGTGGGTAGTGCCCTAGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC019480
• Insert Size: 1098 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC019480, AAH19480
• RefSeq Size: 1423 bp
• RefSeq ORF: 1097 bp
• Locus ID: 68730
• Cytogenetics: 11 E2
• Gene Summary: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.[UniProtKB/Swiss-Prot Function]