Asrgl1 (BC016106) Mouse Untagged Clone

CAT#: MC217824

Asrgl1 (untagged) - Mouse asparaginase like 1 (cDNA clone MGC:27691 IMAGE:4921265), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Asrgl1
• Synonyms: ALP, ALP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC016106
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCTAGTATCATGGATGGGAAGGACCTGTCTGCAGGTGCAGTGTCTGCCGTGCGCTGTATCGCAA
  ATCCCGTTAAACTTGCACGGCTTGTTATGGAAAAGACACCTCATTGCTTTCTGACTGGCCATGGTGCAGA
  GAAATTTGCAGAAGACATGGGGATTCCACAGGTCCCTGTAGAAAAACTGATAACTGAGAGAACCAAGAAG
  CACCTGGAGAAAGAGAAGCTTGAGAAGGGAGCACAGAATGCTGACTGCCCTAAAAACTCAGGAACTGTGG
  GTGCTGTTGCCTTGGACTGCAGAGGAAACTTGGCTTACGCAACCTCTACTGGGGGGATTGTCAATAAAAT
  GGTTGGCCGAGTTGGAGACTCGCCTTGCATAGGAGCTGGAGGTTACGCAGATAATAACCTTGGAGCCGTT
  TCAACCACAGGACATGGGGAAAGTATCCTGAAGGTGAATCTGGCCAGACTTGCCCTCTTCCATGTAGAGC
  AAGGAAAGACCGTAGAGGAGGCTGCTCAGTTGGCATTGGATTACATGAAGTCAAAACTCAAAGGTCTAGG
  TGGCCTCATCTTGGTCAACAAAACAGGAGACTGGGTGGCAAAGTGGACCTCTGCCTCCATGCCCTGGGCA
  GCGGTGAAGAATGGCAAGCTGCAGGCCGGCATTGACCTCTGTGAGACCAGGACAAGGGACCTACCTTGCT
  AG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC016106
• Insert Size: 702 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC016106, AAH16106
• RefSeq Size: 1723 bp
• RefSeq ORF: 701 bp
• Locus ID: 66514
• Cytogenetics: 19 A
• Gene Summary: Has both L-asparaginase and beta-aspartyl peptidase activity. May be involved in the production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. Is highly active with L-Asp beta-methyl ester. Besides, has catalytic activity toward beta-aspartyl dipeptides and their methyl esters, including beta-L-Asp-L-Phe, beta-L-Asp-L-Phe methyl ester (aspartame), beta-L-Asp-L-Ala, beta-L-Asp-L-Leu and beta-L-Asp-L-Lys. Does not have aspartylglucosaminidase activity and is inactive toward GlcNAc-L-Asn. Likewise, has no activity toward glutamine.[UniProtKB/Swiss-Prot Function]