Pftk1 (BC068134) Mouse Untagged Clone

CAT#: MC217730

Pftk1 (untagged) - Mouse PFTAIRE protein kinase 1 (cDNA clone MGC:95907 IMAGE:6834018), (10ug)


  "BC068134" in other vectors (3)

Reconstitution Protocol

USD 503.00

2 Weeks*

Size
    • 10 ug

Product Images

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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Pftk1
Synonyms mKIAA0834; Pftk1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC068134
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTACCCGGAACTGCCAGGGGACAGATTCAGTGATCAAGCACCTGGACACAATTCCTGAAGACAAGA
AAGTCAGGGTTCAGAGGACGCAGAGCACTTTTGACCCATTTGAGAAACCAGCCAACCAAGTCAAAAGGGT
CCATTCTGAGAACAATGCATGCATTAACTTTAAATCCTCCTCTGCTGGCAAAGAGTCACCTAAAGTTCGG
CGGCACTCCAGCCCCAGCTCGCCAACGAGTCCCAAATTTGGAAAAGCTGACTCATACGAAAAACTGGAAA
AACTGGGGGAAGGATCTTATGCAACAGTGTACAAAGGGAAAAGCAAAGTGAATGGGAAGCTGGTGGCTCT
AAAGGTGATCCGGCTGCAGGAAGAAGAGGGCACACCTTTCACAGCCATCAGGGAAGCTTCCCTGTTGAAA
GGACTAAAGCACGCCAACATCGTGTTGCTTCACGACATCATCCACACTAAGGAAACCCTGACCCTTGTCT
TTGAATACGTGCACACTGATTTATGTCAGTACATGGACAAGCACCCTGGAGGACTCCATCCAGATAATGT
GAAGTTGTTTTTATTTCAGCTGCTGCGAGGACTGTCTTACATCCACCAGCGTTATATTTTGCACAGAGAC
CTGAAACCGCAGAACCTTCTCATCAGCGATACGGGGGAGTTGAAGCTGGCAGATTTCGGTCTGGCAAGAG
CAAAATCCGTCCCTAGCCACACATACTCCAATGAAGTGGTTACCTTGTGGTACAGACCTCCAGATGTTCT
TCTGGGCTCTACAGAATATTCCACCTGCCTTGACATGTGGGGAGTTGGCTGTATCTTCGTTGAGATGATC
CAAGGAGTTGCTGCGTTTCCAGGAATGAAAGACATTCAGGATCAACTTGAACGGATATTTCTGGTTCTTG
GAACACCGAATGAGGACACGTGGCCTGGAGTTCATTCTTTACCACATTTTAAGCCAGAACGCTTTACCGT
GTACAGCTCTAAAAGCCTTAGACAAGCATGGAATAAGCTCAGCTATGTAAATCATGCTGAAGACTTGGCC
TCCAAGCTTCTCCAGTGTTCCCCAAAGAACAGGCTATCAGCACAGGCCGCCTTGAGCCATGAGTATTTCA
GCGATCTGCCTCCACGGCTATGGGAGCTGACTGATATGTCTTCTATTTTTACCGTCCCAAATGTGAGATT
GCAACCAGAAGCTGGAGAGAGCATGAGGGCCTTTGGAAAAAACAATAGTTATGGGAAAAGCCTATCGAAC
AGCAAACACTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Restriction Sites SgfI-MluI     
ACCN BC068134
Insert Size 1272 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC068134, AAH68134
RefSeq Size 5127 bp
RefSeq ORF 1271 bp
Locus ID 18647
Cytogenetics 5 2.61 cM
Gene Summary Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport (By similarity).[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.