Otc (BC024893) Mouse Untagged Clone

CAT#: MC217727

Otc (untagged) - Mouse ornithine transcarbamylase (cDNA clone MGC:28946 IMAGE:4196455), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Otc
• Synonyms: AI265390; Sf; spf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217727 representing BC024893
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTCTAATTTGAGGATCCTGCTCAACAATGCAGCTCTTAGAAAGGGTCACACTTCTGTGGTTCGAC
  ATTTTTGGTGTGGGAAGCCAGTCCAAAGTCAAGTACAGCTGAAAGGCCGTGACCTCCTCACCTTGAAGAA
  CTTCACAGGAGAGGAGATTCAGTACATGCTATGGCTCTCTGCAGATCTGAAATTCAGGATCAAGCAGAAA
  GGAGAATATTTACCTTTATTGCAAGGGAAATCCTTAGGAATGATTTTTGAGAAAAGAAGTACTCGAACAA
  GACTGTCCACAGAAACAGGCTTTGCTCTGCTGGGAGGACACCCTTCCTTTCTTACCACACAAGACATTCA
  CTTGGGTGTGAATGAAAGTCTCACAGACACCGCTCGTGTCTTATCTAGCATGACAGATGCAGTGTTAGCT
  CGAGTGTATAAACAATCAGATCTGGACACCCTGGCTAAAGAAGCATCCATCCCAATTGTCAATGGACTGT
  CAGACTTGTATCATCCTATCCAGATCCTGGCTGATTACCTTACACTCCAGGAACACTATGGCTCTCTCAA
  AGGTCTTACCCTCAGCTGGATAGGGGATGGGAACAATATCTTGCACTCTATCATGATGAGTGCTGCAAAA
  TTCGGGATGCACCTTCAAGCAGCTACTCCAAAGGGTTATGAGCCAGATCCTAATATAGTCAAGCTAGCAG
  AGCAGTATGCCAAGGAGAATGGTACCAAGTTGTCAATGACAAATGATCCACTGGAAGCAGCACGTGGAGG
  CAATGTATTAATTACAGATACTTGGATAAGCATGGGACAAGAGGATGAGAAGAAAAAGCGTCTTCAAGCT
  TTCCAAGGTTACCAGGTTACGATGAAGACTGCCAAAGTGGCTGCGTCTGACTGGACATTTTTACACTGTT
  TGCCTAGAAAGCCAGAAGAAGTGGATGATGAAGTATTTTATTCTCCACGGTCATTAGTGTTCCCAGAGGC
  AGAGAATAGAAAGTGGACAATCATGGTCTCACTGAGATCTCACCATGCCCCTTGCCTTGGATCACTCTTC
  TTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC024893
• Insert Size: 1056 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC024893, AAH24893
• RefSeq Size: 3011 bp
• RefSeq ORF: 1055 bp
• Locus ID: 18416
• Cytogenetics: X 4.66 cM
• Gene Summary: Catalyzes the second step of the urea cycle, the condensation of carbamoyl phosphate with L-ornithine to form L-citrulline. The urea cycle ensures the detoxification of ammonia by converting it to urea for excretion.[UniProtKB/Swiss-Prot Function]