Rpe65 (NM_029987) Mouse Untagged Clone

CAT#: MC217576

Rpe65 (untagged) - Mouse retinal pigment epithelium 65 (Rpe65), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rpe65
• Synonyms: 65kDa; A930029L06Rik; LCA2; Mord1; rd12; RP20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217576 representing NM_029987
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTATCCAAATTGAACACCCTGCTGGTGGCTACAAGAAACTATTTGAAACTGTGGAGGAACTGTCCT
  CACCACTAACAGCTCATGTCACAGGCAGGATTCCCCTCTGGCTCACTGGCAGTCTCCTCCGATGTGGGCC
  AGGGCTCTTTGAAGTTGGATCTGAGCCTTTCTATCACCTGTTTGATGGACAAGCCCTTTTGCACAAGTTT
  GACTTCAAGGAGGGCCATGTCACATACCACAGAAGATTCATCCGCACTGATGCTTATGTTCGAGCAATGA
  CTGAGAAGAGGATTGTCATAACAGAATTTGGCACCTGTGCTTTCCCAGACCCCTGCAAGAATATATTTTC
  CAGGTTTTTTTCTTACTTTAAAGGAGTAGAGGTTACTGACAATGCCCTTGTAAATATCTACCCAGTGGGA
  GAAGATTACTATGCATGCACAGAGACCAACTTTATCACAAAGATTAACCCAGAGACCTTGGAGACAATTA
  AGCAGGTTGATCTTTGCAACTATATTTCTGTCAATGGTGCCACTGCTCATCCACATATTGAAAGTGATGG
  AACAGTTTACAACATTGGGAATTGCTTTGGAAAAAATTTTACAGTTGCCTACAACATTATTAAGATCCCT
  CCACTGAAAGCAGACAAGGAAGATCCAATAAACAAGTCAGAAGTTGTTGTGCAGTTCCCCTGCAGTGATC
  GTTTCAAGCCATCTTATGTACACAGTTTTGGTCTGACTCCCAACTATATCGTTTTTGTGGAGACTCCAGT
  CAAAATTAACCTTTTCAAGTTTCTTTCTTCGTGGAGTCTTTGGGGAGCCAACTACATGGACTGTTTCGAG
  TCCAATGAAAGCATGGGGGTTTGGCTTCATGTTGCTGATAAAAAAAGAAGAAAATACTTCAATAACAAAT
  ACAGGACTTCCCCTTTCAATCTCTTCCATCATATCAATACTTATGAAGACAATGGATTTCTGATTGTGGA
  TCTCTGTTGCTGGAAAGGGTTTGAATTTGTTTATAATTACTTATATTTAGCCAATTTACGTGAGAATTGG
  GAAGAAGTTAAAAGAAATGCTATGAAGGCTCCTCAGCCTGAAGTCAGGAGATATGTACTTCCTTTGACAA
  TTGACAAGGTCGACACAGGCAGAAATTTAGTCACACTGCCCCATACAACTGCCACAGCCACTCTGCGCAG
  TGATGAGACCATATGGCTGGAACCTGAGGTTCTCTTTTCAGGGCCTCGTCAAGCCTTTGAATTTCCTCAA
  ATCAATTACCAGAAATTTGGAGGGAAACCTTATACTTATGCATACGGACTTGGGTTGAATCACTTTGTTC
  CTGACAAGCTCTGTAAGATGAACGTCAAAACTAAAGAAATCTGGATGTGGCAAGAGCCAGATTCTTACCC
  ATCTGAACCCATCTTTGTTTCTCAACCAGATGCTCTGGAAGAAGATGATGGTGTGGTTCTGAGTGTGGTG
  GTGAGCCCTGGGGCAGGGCAAAAGCCTGCATATCTCCTGGTTCTGAATGCCAAAGACTTGAGTGAAATTG
  CCAGGGCTGAAGTGGAGACTAATATCCCTGTGACCTTCCATGGACTGTTCAAAAGATCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029987
• Insert Size: 1602 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029987.2, NP_084263.2
• RefSeq Size: 1862 bp
• RefSeq ORF: 1602 bp
• Locus ID: 19892
• UniProt ID: Q91ZQ5
• Cytogenetics: 3 82.52 cM
• Gene Summary: Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore (PubMed:15765048, PubMed:9843205, PubMed:23407971, PubMed:28500718). Essential for the production of 11-cis retinal for both rod and cone photoreceptors (PubMed:17251447). Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT (By similarity).[UniProtKB/Swiss-Prot Function]