Was (NM_009515) Mouse Untagged Clone

CAT#: MC217446

Was (untagged) - Mouse Wiskott-Aldrich syndrome homolog (human) (Was), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Was
• Synonyms: U42471; Wasp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217446 representing NM_009515
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATAGTGGCCCTGGCCCTGTAGGAGGCAGGCCTGGGGGCCGAGGGGGACCAGCCGTTCAGCAGAACA
  TTCCTTCCAACCTCCTCCAGGACCATGAAAACCAGAGACTCTTTGAGCTTCTTGGCCGAAAATGCTGGAC
  ACTGGCTACCACAGTTGTTCAGCTCTACCTGGCACTGCCCCCTGGAGCTGAGCACTGGACCATGGAACAC
  TGCGGGGCTGTGTGCTTCGTGAAGGATAACCCTCAGAAGTCCTACTTCATCCGCCTTTATGGCCTACAGG
  CTGGTCGGCTACTCTGGGAACAGGAGCTGTACTCTCAGCTGGTTTATCTCACTCCCACCCCGTTCTTCCA
  CACTTTTGCTGGAGATGACTGTCAAGTAGGACTGAACTTTGCGGATGAGAGTGAAGCCCAGGCCTTCCGG
  GCCTTGGTGCAGGAGAAGATACAAAAAAGGAATCAGAGGCAAAGCGGAGAAAGACGCCAGCTACCACCAC
  CACCAGCACCAATCAATGAGGAGAGAAGAGGAGGGCTCCCACCTGTGCCCCCACACCCGGGTGGAGATCA
  TGGGGGCCCATCAGGTGGTCCACTATCTCTAGGACTTGTGACGGTCGACATTCAGAACCCTGACATCACA
  AGTTCACGATACCGTGGGCTCCCTGCACCTGGCCCTGGCCCAACTGATAAGAAACGCTCAGGGAAAAAGA
  AGATCAGCAAAGCTGATATCGGAGCACCGAGTGGATTCAAACATGTCAGCCACGTGGGCTGGGATCCCCA
  GAATGGATTTGATGTGAACAACCTAGACCCGGATCTGCGGAGCTTGTTCTCCAGGGCAGGAATCAGCGAG
  GCCCAGCTCACTGACGCAGAGACCTCCAAGCTCATCTACGATTTTATTGAGGACCAGGGAGGTCTAGAGG
  CTGTCCGGCAGGAGATGAGGCGCCAAGAGCCACTCCCACCACCTCCGCCGCCATGCAGAGGAGGAGGAGG
  AGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGCCAGCCTCTGAGACCTCCTGTTGTGGGG
  AGTAATAAGGGTCGCTCAGGTCCACTGCCCCCTGTACCTATGGGGGGTGCCCCACCTCCACCAACACCAC
  GAGGGCCCCCACCACCAGGCCGAGGGGGTCCTCCTCCACCACCCCCTCCAGCCACTGGACGATCTGGACC
  ACCACCTCCTCCACTCCCTGGAGCTGGGGGACCACCAGCACCGCCACCACCACCACCACCACCACCACCT
  CCACCCTGCCCTGGGAGTGGGCCCGCCCCTCCCCCGCTCCCTCCTACTCCAGTGTCTGGGGGGAGCCCAG
  CACCTGGTGGGGGCCGGGGTGCACTTTTGGACCAAATCCGGCAGGGAATTCAGCTGAACAAGACCCCTGG
  AGCTCTAGAGAACTCAGTACAGCAACCACCCGCGCAGCAGTCAGAAGGCCTAGTAGGTGCCCTGATGCAT
  GTCATGCAGAAGAGGAGTAGAGTCATCCATTCCTCAGATGAAGGGGAGGATCAGACCGGCGAGGATGAAG
  AGGATGATGAATGGGATGACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009515
• Insert Size: 1563 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009515.2, NP_033541.1
• RefSeq Size: 2094 bp
• RefSeq ORF: 1563 bp
• Locus ID: 22376
• UniProt ID: P70315
• Cytogenetics: X 3.65 cM
• Gene Summary: Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).[UniProtKB/Swiss-Prot Function]