Sgk1 (NM_001161845) Mouse Untagged Clone

CAT#: MC217438

Sgk1 (untagged) - Mouse serum/glucocorticoid regulated kinase 1 (Sgk1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sgk1
• Synonyms: Sg; Sgk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217438 representing NM_001161845
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAAACAAAGACATGAATGGATTCCCGGTCAAGAAATGCTCAGCGTTCCAATTTTTTAAGAAACGGG
  TACGAAGATGGATCAAGAGCCCCATGGTCAGCGTGGACAAGCATCAGAGCCCCAACTTGAAGTACACTGG
  CCCTGCTGGGGTGCATCTTCCCCCTGGGGAGTCAGACTTTGAGGCCATGTGTCAATCATGCCTGGGTGAC
  CATGCTTTCCAAAGGGGGATGCTCCCTCCAGAGGAGTCCTGTTCCTGGGAGATCCAACCTGGGTGTGAAG
  TGAAAGAACAATGTAATCATGCCAACATCCTGACCAAGCCGGACCCAAGAACCTTCTGGACTAATGATGA
  TGCAGCTTTTATGAAACAGAGAAGGATGGGCCTGAACGATTTTATTCAGAAGATTGCCAGCAACACCTAT
  GCATGCAAACACGCTGAAGTTCAGTCCATTTTGAAAATGTCCCATCCTCAGGAGCCGGAGCTTATGAACG
  CTAACCCCTCTCCTCCGCCAAGTCCCTCTCAACAAATCAACCTGGGTCCGTCCTCCAACCCTCACGCCAA
  ACCCTCCGACTTTCACTTCTTGAAAGTGATCGGAAAGGGCAGTTTTGGAAAGGTTCTTCTGGCTAGGCAC
  AAGGCAGAAGAAGTATTCTATGCAGTCAAAGTTTTACAGAAGAAAGCCATCCTGAAGAAGAAAGAGGAGA
  AGCATATTATGTCAGAGCGGAATGTTCTGTTGAAGAATGTGAAGCACCCTTTCCTGGTGGGCCTTCACTT
  CTCATTCCAGACCGCTGACAAGCTCTACTTTGTCCTGGACTACATTAATGGTGGAGAGCTGTTCTACCAT
  CTCCAGAGGGAGCGCTGCTTCCTGGAACCACGGGCTCGATTCTACGCAGCTGAAATAGCCAGTGCCCTGG
  GCTATCTGCACTCCCTAAACATCGTTTATAGAGACTTAAAACCTGAGAATATTCTCCTAGACTCCCAGGG
  GCACATCGTCCTCACTGACTTTGGGCTCTGCAAAGAGAATATTGAGCATAACGGGACAACATCTACCTTC
  TGTGGCACGCCTGAGTATCTGGCTCCTGAGGTCCTCCATAAGCAGCCGTATGACCGGACGGTGGACTGGT
  GGTGTCTTGGGGCTGTCCTGTATGAGATGCTCTACGGCCTGCCCCCGTTTTATAGCCGGAACACGGCTGA
  GATGTACGACAATATTCTGAACAAGCCTCTCCAGTTGAAACCAAATATTACAAACTCGGCAAGGCACCTC
  CTGGAAGGCCTCCTGCAGAAGGACCGGACCAAGAGGCTGGGTGCCAAGGATGACTTTATGGAGATTAAGA
  GTCATATTTTCTTCTCTTTAATTAACTGGGATGATCTCATCAATAAGAAGATTACACCCCCATTTAACCC
  AAATGTGAGTGGGCCCAGTGACCTTCGGCACTTCGATCCCGAGTTTACCGAGGAGCCGGTCCCCAGCTCC
  ATCGGCAGGTCCCCTGACAGCATCCTTGTCACGGCCAGTGTGAAGGAAGCAGCAGAAGCCTTCCTCGGCT
  TCTCCTATGCACCTCCTGTGGATTCCTTCCTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001161845
• Insert Size: 1575 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161845.2, NP_001155317.2
• RefSeq Size: 3121 bp
• RefSeq ORF: 1575 bp
• Locus ID: 20393
• UniProt ID: Q9WVC6
• Cytogenetics: 10 A3
• Gene Summary: This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. This enzyme is activated by protein phosphorylation and degraded via the ubiquitination and proteasome pathway. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene was identified on chromosome 12. [provided by RefSeq, Sep 2009]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).