Cyp1a1 (NM_009992) Mouse Untagged Clone

CAT#: MC217415

Cyp1a1 (untagged) - Mouse cytochrome P450, family 1, subfamily a, polypeptide 1 (Cyp1a1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cyp1a1
• Synonyms: AHH; AHRR; CP11; Cyp1a2; CYPIA1; P450-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217415 representing NM_009992
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTCCATGTATGGACTTCCAGCCTTCGTGTCAGCCACAGAGCTGCTCCTGGCTGTCACCGTATTCT
  GCCTTGGATTCTGGGTGGTCAGAGCCACAAGAACCTGGGTTCCCAAAGGCCTGAAGACTCCACCAGGGCC
  CTGGGGCTTGCCCTTCATTGGTCACATGCTGACTGTGGGGAAGAACCCACATCTGTCACTGACACGGCTG
  AGTCAGCAGTATGGGGACGTGCTGCAGATCCGCATCGGCTCCACTCCTGTGGTGGTGCTGAGCGGCCTGA
  ACACCATCAAGCAGGCCCTGGTGAGGCAGGGAGATGACTTCAAGGGCCGGCCAGACCTCTACAGCTTCAC
  ACTTATCACTAATGGCAAGAGCATGACTTTTAACCCAGACTCTGGACCCGTGTGGGCTGCCCGCCGGCGC
  CTGGCCCAGAATGCCCTGAAGAGCTTCTCCATAGCCTCGGACCCGACGTCAGCATCCTCTTGCTACTTGG
  AGGAGCACGTGAGCAAGGAGGCTAACTATCTCGTCAGCAAACTTCAGAAGGTGATGGCAGAGGTTGGCCA
  CTTTGACCCTTACAAGTATTTGGTCGTGTCAGTAGCCAATGTCATCTGTGCCATATGCTTTGGCCAACGT
  TATGACCATGATGACCAAGAGCTGCTCAGCATAGTCAATCTGAGCAATGAGTTTGGGGAGGTTACTGGCT
  CTGGATACCCAGCTGACTTCATTCCTGTCCTCCGTTACCTGCCTAACTCTTCCCTGGATGCCTTCAAGGA
  CTTGAATGATAAGTTCTACAGCTTCATGAAGAAGTTAATCAAAGAGCACTACAGGACATTTGAGAAGGGC
  CACATCCGGGACATCACAGACAGCCTCATTGAGCATTGTCAGGACAGGAAGCTGGACGAGAATGCCAATG
  TCCAGCTGTCAGATGATAAGGTCATCACGATTGTTTTGGACCTCTTTGGAGCTGGGTTTGACACAGTCAC
  AACTGCTATCTCGTGGAGCCTCATGTACCTGGTAACCAACCCTAGGGTACAGAGAAAGATCCAGGAGGAA
  CTAGACACAGTGATTGGCAGAGATCGGCAGCCCCGGCTTTCTGACAGACCTCAGCTGCCCTATCTGGAGG
  CCTTCATTCTGGAGACCTTCCGGCATTCATCCTTCGTCCCCTTCACCATCCCCCACAGCACCACAAGAGA
  TACAAGTCTGAATGGCTTCTATATCCCCAAGGGGTGCTGTGTCTTTGTGAACCAGTGGCAGGTTAACCAT
  GACCGGGAACTGTGGGGTGACCCAAACGAGTTCCGGCCTGAAAGGTTTCTCACCCCCAGCGGCACTCTGG
  ACAAGCGCTTGAGTGAGAAGGTCACTCTCTTTGGTTTGGGCAAGCGAAAGTGCATCGGAGAGACCATTGG
  CCGATCGGAGGTCTTTCTCTTCCTGGCCATCTTGCTGCAGCAAATAGAATTTAAGGTGTCTCCAGGGGAG
  AAGGTGGATATGACTCCTACCTATGGGCTGACTTTAAAACACGCCCGCTGTGAACACTTCCAAGTGCAGA
  TGCGGTCTTCTGGTCCTCAGCATCTTCAGGCTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009992
• Insert Size: 1575 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009992.4, NP_034122.1
• RefSeq Size: 2716 bp
• RefSeq ORF: 1575 bp
• Locus ID: 13076
• UniProt ID: P00184
• Cytogenetics: 9 31.34 cM
• Gene Summary: A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15alpha and C16alpha positions. Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation. Catalyzes the epoxidation of double bonds of certain PUFA. Converts arachidonic acid toward epoxyeicosatrienoic acid (EET) regioisomers, 8,9-, 11,12-, and 14,15-EET, that function as lipid mediators in the vascular system. Displays an absolute stereoselectivity in the epoxidation of eicosapentaenoic acid (EPA) producing the 17(R),18(S) enantiomer. May play an important role in all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.