Uspl1 (NM_001013378) Mouse Untagged Clone

CAT#: MC217361

Uspl1 (untagged) - Mouse ubiquitin specific peptidase like 1 (Uspl1), transcript variant 4, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Uspl1
• Synonyms: E430026A01Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217361 representing NM_001013378
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGATGATTCTCTGAAGATTGGAAATGGTTTGCCACTGGTTGGACCAGGGACTGATGTAGGGATAT
  CTTCACTCCCCATGCTGGGGTACTTGGGAAAAAATTATGCTTCAGCTAAAGTCACAACAGACGGGCATTG
  CCCTGCTTGTAGAGCGAAAGGCAAGCTAAGTGCCTTAAAACCTTACCGCATTAGCTTCCAAGAATCTGTC
  TTTCTGTGTGAGGATCTTCAGTGCATTTATCCTTTGGGCTCTGAGTCACTTACTAATTTAATTTCTCCTG
  ATTCAGAAGATTGTCCCACTCCAAGTAAGCCTCAAAAACGAAAAAGATTGGAAACCAACTGTAGGAATTC
  ACCTCTTCCAGTACATTCTAAAAAGACTAGAAGTCACATTGTCACTGATAGTGAACCAATTGTGAATGGT
  AAATATAATGGAGAAGTGTGTGATGACTTCTCAGCAAGCTTCCCAGATACCAGTGCTCACCAGGATCCTG
  CCAGCACAGCTGCTTCTGTGGAGCAGAGTGAGGCTTTGGAGGCTGATGATGTGGTTGTGGCCGCTACAGA
  AGATCCTGCTACAGTTAGTGTCACATCTGAGCTGGAAATGCCAGCTAAGAGCAGATGTTTACCACTTTGC
  CAGACTCTGTGTGTCCAGTGGAAAAACACCCAGGCCCTGTGCTGGTTAGACTGTATCCTGTCAGCCCTGG
  TGCACTTGGAGGTGTTGAGGAAGACTGTGCTGGAGGCGTGCTCCAGGGAGGAGTGTGTATTTGGAAGGCT
  GTTTGAAATGTACCACCAAGCAGATGAGCTGCTGCACACCCATCACCTGCATGGCGTCACAGGTGAAGAT
  TGTAAAAAATTGACATCAGAAATATTTACAGAAATAGATACCTGTTTGAATAAAGTTAGAGATGAAATTT
  TTGCTAAACTTCAACCGAAGCTTAGATGCACATTAGGTGACATGGAAAGTCCTGTGTTTGCACTTCCTGT
  ACTGTTAAAGCTTGAACCCCATGTTGAAAGCCTCTTTACATATTCTTTTTCTTGGAATTTTGAATGTTCC
  CATTGTGGACACCAGTACCAAAACAGGTGTGTGAAGAGTCTGGTCACCTTTACCAATATTGTTCCTGAGT
  GGCATCCACTCAATGCTGCCCATTTTGGTCCATGTAACAGCTGCAACAGTAAATCACAAATAAGAAAAAT
  GGTGTTGGAAAGAGCGTCGCCCATACTCATGCTGCACTTCGTGGAGGGCTTACCAAGGAGGGATTTGCAA
  CACTATGCCTTCCACTTTGAAGGAAGCCTTTATCAAGTCACGTCGGTAATACAGTACCAAGCAAATAACC
  ATTTTATAACATGGTTTTTAGATGCTGATGGGAGTTGGCTGGAGTGTGATGACCTAAAGGGTCCCTGTGC
  TAAAAGGCACGTGACCTGTGAAGTGCCAGCTTCAGAGACACATATCGTTATTTGGGAAAGAAAATCCCAG
  GTGCCAATTGAAGAAGCTGCCTGCCTTCCATGCATGAAGCCAAATGTGCAGCCTGTATCAGGAATTGAAG
  TCCCTGGCAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001013378
• Insert Size: 1554 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013378.2, NP_001013396.2
• RefSeq Size: 3272 bp
• RefSeq ORF: 1554 bp
• Locus ID: 231915
• UniProt ID: Q3ULM6
• Cytogenetics: 5 G3
• Gene Summary: SUMO-specific isopeptidase involved in protein desumoylation. Specifically binds SUMO proteins with a higher affinity for SUMO2 and SUMO3 which it cleaves more efficiently. Also able to process full-length SUMO proteins to their mature forms (By similarity). Plays a key role in RNA polymerase-II-mediated snRNA transcription in the Cajal bodies (By similarity). Is a component of complexes that can bind to U snRNA genes (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR and contains alternate splice sites in the 3' coding region, resulting in a frameshift and an early stop codon, compared to variant 1. It encodes isoform C, which is shorter and has a distinct C-terminus, compared to isoform A. Variants 4 and 9 both encode the same isoform (C).