Ptger4 (NM_008965) Mouse Untagged Clone

CAT#: MC217286

Ptger4 (untagged) - Mouse prostaglandin E receptor 4 (subtype EP4) (Ptger4), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ptger4
• Synonyms: EP4; Ptgerep4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217286 representing NM_008965
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGGTTGGAGGTACCATTCCTAGATCGAACCGTGAGCTCCAACGCTGTGTGTTACTAACCACCA
  CCATCATGTCCATCCCCGGAGTCAACGCGTCCTTCTCCTCCACTCCGGAGAGGCTGAACAGCCCGGTGAC
  CATTCCCGCAGTGATGTTCATCTTCGGGGTGGTGGGCAACCTGGTGGCCATCGTAGTATTGTGCAAGTCG
  CGCAAGGAGCAGAAAGAGACGACCTTTTACACTCTAGTATGTGGGCTGGCTGTCACTGACCTTCTGGGCA
  CCTTGTTGGTAAGCCCGGTGACCATCGCCACATACATGAAGGGCCAGTGGCCCGGAGACCAGGCACTGTG
  TGACTATAGCACCTTCATCCTACTTTTCTTCGGTCTGTCGGGTCTCAGCATCATCTGTGCCATGAGCATC
  GAGCGCTACCTGGCCATCAACCACGCCTACTTCTACAGCCACTACGTGGACAAGCGGCTGGCCGGCCTCA
  CACTCTTCGCCATCTATGCATCTAACGTGCTGTTCTGCGCGCTGCCCAACATGGGCCTGGGCAGATCCGA
  GCGGCAGTACCCGGGCACCTGGTGCTTCATCGACTGGACCACCAACGTAACGGCCTACGCCGCCTTCTCT
  TACATGTACGCCGGCTTCAGCTCCTTCCTCATCCTTGCCACCGTGCTCTGCAACGTGCTGGTGTGCGGCG
  CGCTGCTCCGCATGCACCGCCAGTTCATGCGCCGCACCTCGTTGGGCACGGAGCAGCACCATGCGGCTGC
  CGCCGCCGCGGTAGCTTCGGTGGCCTGTCGGGGCCACGCTGGGGCCTCCCCAGCCCTGCAGCGCCTCAGC
  GACTTTCGCCGCCGCAGGAGTTTCCGGCGCATCGCGGGTGCGGAGATCCAGATGGTCATCTTACTCATCG
  CCACCTCTCTGGTGGTGCTCATCTGCTCCATTCCGCTCGTGGTGCGAGTGTTCATTAACCAGTTATATCA
  GCCAAACGTGGTGAAAGACATCAGCAGAAACCCAGATTTGCAGGCCATCAGGATTGCTTCTGTGAACCCC
  ATCCTGGACCCCTGGATTTACATCCTTCTTCGGAAGACTGTGCTCAGTAAAGCCATAGAGAAGATCAAGT
  GCCTCTTCTGCCGCATTGGCGGTTCCGGCAGAGACAGCTCGGCCCAGCACTGCTCAGAGAGTCGGAGGAC
  ATCTTCCGCCATGTCCGGCCACTCTCGCTCCTTCCTCGCCCGGGAGTTAAAGGAGATCAGCAGCACGTCC
  CAGACCCTCCTGTACCTGCCAGACCTGACTGAAAGCAGCCTCGGAGGCAGGAATTTGCTTCCAGGTTCGC
  ATGGCATGGGCCTGACCCAAGCAGACACCACCTCGCTGAGAACTTTGCGAATTTCCGAGACCTCAGACTC
  CTCCCAGGGCCAGGACTCTGAGAGTGTCCTGTTGGTGGATGAGGTTAGTGGGAGCCACAGAGAGGAGCCT
  GCCTCTAAAGGAAACTCTCTGCAAGTCACATTCCCCAGTGAAACTCTGAAATTATCTGAAAAATGTATAT
  AG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_008965
• Insert Size: 1542 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008965.2, NP_032991.1
• RefSeq Size: 3264 bp
• RefSeq ORF: 1542 bp
• Locus ID: 19219
• UniProt ID: P32240
• Cytogenetics: 15 1.99 cM
• Gene Summary: Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) encodes the longer isoform (2). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.