Chrng (NM_009604) Mouse Untagged Clone

CAT#: MC217273

Chrng (untagged) - Mouse cholinergic receptor, nicotinic, gamma polypeptide (Chrng), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Chrng
• Synonyms: Achr-3; Acrg
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217273 representing NM_009604
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAGGGGGCCAGAGACCTCAGCTCCTCTTGCTGCTGTTGGCTGTCTGCCTGGGGGCCCAGAGCCGCA
  ACCAAGAGGAGCGTCTGCTTGCGGACCTGATGCGAAACTACGACCCCCACCTGCGGCCGGCTGAGCGCGA
  CTCAGATGTGGTCAATGTCAGCCTGAAGCTTACCTTGACCAACCTCATCTCCCTGAATGAACGAGAGGAG
  GCCCTCACAACTAACGTCTGGATAGAGATGCAATGGTGCGACTATCGCCTGCGCTGGGACCCAAAAGACT
  ACGAAGGCCTGTGGATATTGAGGGTGCCATCTACTATGGTCTGGCGGCCAGATATCGTCCTGGAGAACAA
  TGTGGACGGTGTCTTCGAGGTGGCTCTCTACTGCAATGTCCTCGTGTCCCCCGACGGTTGTATCTACTGG
  CTGCCGCCTGCCATCTTCCGCTCCTCCTGCTCCATCTCTGTCACCTACTTCCCCTTCGATTGGCAGAACT
  GTTCCCTCGTCTTCCAATCCCAGACTTACAGCACCAGTGAGATCAACTTGCAGCTGAGCCAGGAGGATGG
  GCAAGCCATTGAGTGGATCTTCATTGACCCGGAGGCTTTCACAGAGAATGGGGAGTGGGCCATCCGGCAC
  CGACCGGCTAAAATGCTCCTGGACTCCGTGGCTCCTGCAGAGGAGGCGGGCCACCAGAAGGTGGTGTTCT
  ACCTGCTTATCCAGCGCAAGCCCCTCTTCTACGTCATCAACATCATCGCCCCCTGTGTGCTCATCTCCTC
  AGTCGCCATCCTCATCTACTTCCTTCCTGCTAAGGCGGGCGGGCAGAAATGCACAGTGGCCACCAACGTG
  CTCCTGGCCCAGACTGTCTTCCTTTTCCTTGTGGCTAAGAAGGTGCCTGAGACCTCCCAGGCAGTGCCAC
  TCATCAGCAAGTACCTGACCTTCCTCATGGTGGTGACCATCCTCATCGTCGTGAACTCTGTGGTCGTGCT
  CAATGTGTCCTTGCGGTCCCCCCACACACACTCCATGGCCCGTGGGGTCCGCAAGGTGTTCCTGAGGCTC
  CTGCCCCAGCTGTTACGGATGCATGTGCGCCCACTAGCTCCAGCTGCTGTCCAGGATGCCCGGTTCCGAC
  TCCAGAATGGCTCTTCCTCAGGGTGGCCCATCATGGCTCGAGAGGAAGGGGACCTCTGTCTGCCTCGAAG
  CGAACTCCTCTTTAGGCAAAGGCAGCGCAATGGATTAGTGCAGGCAGTATTGGAGAAGCTAGAGAATGGT
  CCAGAAGTGAGGCAGAGCCAGGAGTTCTGTGGCAGCCTGAAGCAAGCCTCCCCAGCCATCCAGGCCTGTG
  TGGATGCCTGTAACCTCATGGCTCGTGCCCGACGCCAGCAGAGTCACTTTGACAGTGGGAACGAGGAGTG
  GTTGCTGGTGGGCCGAGTGCTGGACCGAGTCTGCTTCCTAGCCATGCTCTCCCTCTTCATCTGTGGCACT
  GCTGGCATCTTCCTCATGGCCCACTACAACCAAGTGCCTGACCTGCCGTTCCCCGGAGACCCCCGCCCCT
  ACCTGCCTTTGCCAGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009604
• Insert Size: 1560 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009604.3, NP_033734.3
• RefSeq Size: 1921 bp
• RefSeq ORF: 1560 bp
• Locus ID: 11449
• Cytogenetics: 1 44.07 cM