Kcnc1 (NM_008421) Mouse Untagged Clone

CAT#: MC217139

Kcnc1 (untagged) - Mouse potassium voltage gated channel, Shaw-related subfamily, member 1 (Kcnc1), transcript variant B, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcnc1
• Synonyms: C230009H10Rik; Kcr2-1; KShIIIB; Kv3.1; KV4; NGK2; Shaw
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217139 representing NM_008421
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCAAGGGGACGAGAGCGAGCGCATCGTGATCAACGTGGGCGGCACGCGCCACCAGACGTACCGCT
  CGACGCTGCGCACGCTGCCCGGCACGCGGCTTGCCTGGCTGGCAGAGCCGGACGCCCACAGCCACTTCGA
  CTATGACCCGCGTGCCGACGAGTTCTTCTTCGACCGCCACCCGGGCGTCTTCGCTCACATCCTGAACTAT
  TACCGCACCGGCAAGCTTCACTGCCCGGCCGACGTGTGCGGGCCGCTCTACGAGGAGGAGCTGGCCTTCT
  GGGGCATCGACGAGACGGACGTGGAGCCCTGCTGCTGGATGACCTATCGCCAGCACCGAGACGCTGAGGA
  GGCGCTGGACAGCTTTGGCGGTGCGCCCTTGGACAACAGCGCCGACGACGCGGACGCCGACGGCCCCGGC
  GACTCGGGCGACGGCGAGGACGAGCTGGAGATGACCAAGAGATTGGCACTCAGTGACTCCCCAGATGGCC
  GGCCTGGCGGCTTCTGGCGCCGCTGGCAACCGCGCATCTGGGCGCTGTTCGAGGACCCCTACTCATCCCG
  CTACGCGCGGTATGTGGCCTTTGCCTCCCTCTTCTTCATCCTGGTCTCCATCACAACCTTCTGTCTGGAG
  ACTCACGAGCGCTTCAACCCCATCGTGAACAAGACCGAAATCGAGAACGTTCGAAACGGCACCCAAGTGC
  GGTACTACCGGGAAGCAGAGACGGAGGCCTTCCTCACCTACATCGAGGGCGTCTGCGTGGTCTGGTTCAC
  CTTCGAGTTCCTCATGCGTGTCGTCTTCTGCCCCAACAAGGTGGAATTCATCAAGAACTCCCTCAATATC
  ATTGACTTTGTGGCCATTCTCCCCTTCTACCTGGAGGTGGGCCTAAGCGGCCTGTCCTCAAAAGCCGCCA
  AGGACGTTCTGGGCTTCCTGCGCGTCGTCCGCTTCGTGCGCATCCTGCGCATCTTCAAGCTGACCCGCCA
  CTTCGTGGGCCTGAGGGTCCTGGGCCACACGCTCCGTGCCAGCACCAACGAGTTCCTGCTGCTTATCATC
  TTCCTGGCCCTGGGAGTGCTCATCTTTGCCACCATGATCTACTACGCCGAGAGGATAGGGGCACAGCCCA
  ATGACCCCAGCGCCAGCGAACACACACACTTTAAAAACATCCCCATCGGCTTCTGGTGGGCTGTGGTCAC
  CATGACGACACTGGGCTATGGAGACATGTATCCCCAGACGTGGTCTGGAATGCTGGTGGGAGCCTTGTGT
  GCTCTGGCTGGTGTGCTGACCATTGCCATGCCGGTGCCTGTCATCGTGAACAATTTTGGGATGTACTACT
  CTTTAGCCATGGCTAAGCAGAAACTACCAAAGAAAAAAAAGAAGCATATTCCGCGGCCACCACAGCTGGG
  ATCTCCCAATTATTGTAAATCTGTCGTAAACTCTCCACACCACAGTACTCAGAGTGACACATGCCCGCTG
  GCCCAGGAAGAAATTTTAGAAATTAACAGAGCAGGTAGGAAACCTCTCAGAGGCATGTCGATCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008421
• Insert Size: 1536 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008421.3, NP_032447.1
• RefSeq Size: 7788 bp
• RefSeq ORF: 1536 bp
• Locus ID: 16502
• UniProt ID: P15388
• Cytogenetics: 7 30.1 cM
• Gene Summary: Voltage-gated potassium channel that plays an important role in the rapid repolarization of fast-firing brain neurons. The channel opens in response to the voltage difference across the membrane, forming a potassium-selective channel through which potassium ions pass in accordance with their electrochemical gradient (PubMed:2599109, PubMed:1400413). Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNC2, and possibly other family members as well. Contributes to fire sustained trains of very brief action potentials at high frequency in pallidal neurons.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks two exons and its 3' exon extends past a splice site used in variant 1, resulting in a distinct 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.