Toe1 (NM_026654) Mouse Untagged Clone

CAT#: MC217119

Toe1 (untagged) - Mouse target of EGR1, member 1 (nuclear) (Toe1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Toe1
• Synonyms: 4930584N22Rik; 4933424D16Rik; AI413517
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217119 representing NM_026654
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCGGACAGTGACGATGATGTGCCTCCAGTTCCAACGCCCTCTGACGGTGGTGTCAACAAAAACA
  CACAACCTGCAGAGGAGTTTGTGGTTCGGGTTCCTGTGGTAGACGTGCAGAGTGACAACTTCAAGGAGAT
  ATGGCCATCACTCTTGCTAGCCTTAAAGACAGCTAGCTTTGTGGCTGTGGACACGGAGCTGAGTGGGCTC
  GGGGATAGGAAGAGTTTGCTGAACCAGTGCATTGAGGAACGTTACAAGGCCGTGTGTCATGCTGCCAGGA
  CCCGTTCTGTCCTGTCCCTGGGCCTCGCCTGCTTCAGGCAGCAACCAGATAAGGGTGAAAATTCTTACTT
  GGCTCAAGTGTTCAATCTCACCCTGCTATGCATAGAGGAGTACGTTATAGAACCGAAGTCTGTGCAGTTC
  CTGGTACAGCATGGCTTCAACTTCAACCGGCAGTACGCCCAGGGTATCCCCTACCATAAGGGCAATGACA
  AGGGAGATGAGAGCCAGAGCCAGTCGGTACGGACGCTGTTCCTTGAACTGATTCGGGCCCGACGGCCCCT
  GGTGCTACATAATGGCCTTATAGACTTGGTGTTCCTGTACCAGAATTTCTACGCGCACCTGCCTGAGAAC
  TTGGGGACCTTCACTGCTGACCTGTGTGAGATGTTCCCAGCAGGCATCTATGACACCAAATATGCCGCCG
  AGTTCCATGCCCGATTTGTAGCTTCCTACTTAGAATACGCTTTCCGGAAATGTGAGCGGGAGAATGGGAA
  GCAGCGGGCAGCTGGCAGCCCACATCTTGCCCTGGAATTCTGCAGCTATCCATCTAGCATGAGCGGGCAC
  ATTGACTACCGCTGCTGTATGTCCCCTGGGACCTGCCGTCGTTCTCGTACCACTGGCATCTGTGACAAGT
  TCTCGGCCTATGGCTGGTGTCCTCTGGGACCACAGTGTCCTCAGTCACACGATATTGACCTTATAATTGA
  CACTGATGAGGCTGTGGCAGAAGATAAGCGGCGGAGACGGCGACGTAAGGACAAACGGAAGAGGTCTTTA
  CAGAGCCAACCAGGGACACAGACCCTTGCGGAAGCTGAAGATGGTCCTCCCACAAAACAGGTCTGTGAAG
  ATAGCCTCGAGACTGAGAAAATGGAACAAAAAGTGGCAGAAGGTGAGGCTGGGGATCAGCCTGGCTCCAG
  GGAAGGTCACACAAGTAGCTTGGAGATGGCACATAGGAGGACAAGTGCTGAAACAGCTGATGTGGCCACC
  TCAGAGCTGCTGGTGAACCAAGCCAGCACTAACCCCGTGCCTGGGGACGGACTGCATCGTGCTGGTTTTG
  ACGCCTTTATGACAGGCTATGTGATGGCCTATGTGGGCCTGAGCCAGGGACTTCAACTCTGCAGCTCTGA
  ACCTTGGCTTCCTGAATGTCACAACAAGGTATATCTAAGTGGCAAAACTGTACCCCTCACAGTGACCAAG
  AGCCAGTTTTCCCGTCCATCCAAAGCCCACAACCAGAAAATGAAGCTGGCTTGGGGCAGCAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026654
• Insert Size: 1536 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026654.2, NP_080930.1
• RefSeq Size: 2008 bp
• RefSeq ORF: 1536 bp
• Locus ID: 68276
• UniProt ID: Q9D2E2
• Cytogenetics: 4 D1
• Gene Summary: Inhibits cell growth rate and cell cycle. Induces CDKN1A expression as well as TGF-beta expression. Mediates the inhibitory growth effect of EGR1. Involved in the maturation of snRNAs and snRNA 3'-tail processing.[UniProtKB/Swiss-Prot Function]