Mdh1b (NM_029696) Mouse Untagged Clone

CAT#: MC217029

Mdh1b (untagged) - Mouse malate dehydrogenase 1B, NAD (soluble) (Mdh1b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mdh1b
• Synonyms: 1700124B08Rik; AV255588
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217029 representing NM_029696
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAATTTGTGATCGCGGGTAAAGCAAACTGCCCATATTATGCTAAGGCAGAACTTCTGGCAGACT
  ATTTACAAAAGAATCTTCCTGACTTTCGGATTTTTAAGATCACGCAACATCCTGACAAGTGGGAGGATTG
  GGTGGAAGATGTTTGTGAAAGGAATATGTGGGACCACAGAACCTCCCCCATTATCTGGAGAGAGTTGCTG
  GATCGTGGAGGCAGGGGCTTGCTTTTGGGAGGATACAATGAGTTTCTGGAACATGCCCAGCTTTACTATG
  GTGTCACTTCCAACATGACGACGGAGTTGATGATGGTCATCGCTAAAGAGAACATGCAGACGCATACAGA
  ACAACAGTTGGATAAAGAAACCATGAAAGATCTCATCAGCCCTTTGCAGGTCTGGATTGCCAGTGCAGGT
  ACTTACGTGTGCTGCCACTTAATTCCCCTCTTGCTAAGCGGGGAAGTGTTTGGGATGCACACAGAGATCA
  GCCTGACCCTGTTTGACCAGGAGCAAAGGGAGGACTGCCTCAGAAGCATAGTGATGGAAACCCAGGACTT
  GGCCTCACCAGTGCTCCGCACTGTGTCCTTCTGCACCACGGTAAAGGAGGCCTTCCTTCAGGCCCAGGTC
  ATCATCATCCTGGATGACAGTACAGAGGAAGAGGTGTACAGCCTGGAGAGCTGCCTCCGGAGCAGGGTGC
  CGCTGTGCCGCCTCTACGGCTACCTGATAGAGAAAAACGCTCACAAGTCTGTCAAGGTCATCGTGGGAGG
  GAAAAACTTTGTAAATCTGAAAACAACTTTGCTCATGCAATATGCCCCTAACATCGCGAGCAACATTATC
  GCAGTGGCTCTGGGGGTAGAAGGCCAAGCAAAAGCAGTACTGGCCCGAAAGATGAAAACCACTTCAGCCA
  ACATCAAAGATGTGATAATCTGGGGCAATATCACTGGAAATAACTACGTTGATCTGAGGAAAGCAAAAGT
  TTACAACTATGAGAGTGCTGTCAAGGGGCCTCCTGGACACTATCACTCTGTGTTAAGCTTGATTTTTGAC
  AGAGAGTGGATCACAAAAGAATTTGTGCAGACACTTAAAATCTTGAGCAGCACTGGAAAACAATTTGGAG
  GCATCTTGGCTGCACACAGCATAGCCACCACCCTGAAGTACTGGTATCATGGCTCACCACCTGGAGAGAT
  TGTTTCCTTGGGGGTAATGAGTGAAGGCCAGTTTGACATTCCTGAAGGGATCGTCTTTTCTATGCCAGTG
  AAATTTGAGAATGGAACGTGGGTGGTTCTTACTGATCTTGAAGACATTTCGCTTAGTGAGAAGACACTAA
  GCAGGCTCACAGGCGATCTGATTCAGGAGAAACTTGTTGCATGTGGAGACTTATTAACTTTTCAGCCAAT
  TGAAGAAGATCCCAAAGATAACGAACCTTCTAATACAGGCATGAATGAAGAAAAGGAACAGCCTGGGTCC
  GATGACTCTGATGAAAAAAATGAGGACCAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029696
• Insert Size: 1503 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029696.4, NP_083972.3
• RefSeq Size: 1711 bp
• RefSeq ORF: 1503 bp
• Locus ID: 76668
• UniProt ID: Q5F204
• Cytogenetics: 1 C2