Pdhx (NM_175094) Mouse Untagged Clone

CAT#: MC216992

Pdhx (untagged) - Mouse pyruvate dehydrogenase complex, component X (Pdhx), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pdhx
• Synonyms: AI481367; E3bp; Pdx1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216992 representing NM_175094
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCTCCTGGAGGCTTCACTGTAACCAGCCGCTGCTTCGGTACCTTCTGGGATTTAGCAGCCGTC
  GAAGCCTGGGACTGGCTCAGGGTGCTGCTGCTTGGCCTGTCGACCGTGGAGCAAGTTGGAGGTGGTTTCA
  CAGTACTCAGCTGCTGCAGGCGGATCCTATTAAGGTCCTAATGCCATCACTGTCTCCTACGATGGAGCAA
  GGGAACATTGTGAAATGGCTGAGAAAGGAAGGTGAAGCTGTGAGTGCTGGAGACTCGTTATGTGAAATTG
  AGACGGACAAGGCTGTGGTCACCTTAGATGCAAACGATGATGGCATCTTAGCCAAGATAGTGGTTGAGGA
  AGGTGCTAAAAATATACAGCTGGGTTCACTCATTGCTTTGATGGTTGAAGAAGGGGAAGATTGGAAACAG
  GTGGAAATTCCCAAGGATGTCAGTGCTCCACCTCCAGTCTCAAAACCACCAGCTCCTACTCAGCCCTCTC
  CACAGCCGCAGATTCCTTGTCCCGCCAGGAAGGAGCACAAAGGGACGGCACGGTTCCGCTTAAGTCCAGC
  TGCCCGCAACATTCTGGAAAAACATTCATTGGACGCCAGCCAGGGCACAGCCACAGGCCCTCGCGGGATC
  TTCACTAAAGAAGATGCACTCAAGTTAGTCGAGCTGAAACAGATGGGCAAGATTACTGAATCCAGACCAG
  CCTCAGCTCCTCCACCCTCTCTCTCAGCATCTGTGCCTCCTCAGGCTACAGCCGGGCCATCCTATCCTCG
  GCCAATGACCCCACCAGTATCCATTCCTGGGCAACCGAATGCAGCGGGTACATTCACCGAAATTCCTGCT
  AGCAATATTCGAAGAGTTATCGCTAAGAGGTTAACTGAATCAAAAAGTACTGTGCCTCATGCCTATGCTA
  CTGCTGACTGTGACCTTGGCGCAGTTCTAAAGGTTAGACGAGATCTGGTCAAAGATGACATTAAAGTCTC
  AGTAAATGACTTTATCATCAGAGCAGCAGCTGTTACTCTTAAACAAATGCCTGGGGTTAATGTCACCTGG
  GATGGAGAAGGCCCAAAGCAGCTGCCCTCTGTTGACATTTCAGTGGCTGTGGCAACAGATAAAGGTTTAA
  TTACACCAATCATAAAAGATGCAGCCGCTAAGGGCATTCAGGAGATCGCTGACTCTGTAAAGGTTCTGTC
  GAAGAAAGCACGGGATGGGAAATTGATGCCAGAGGAGTACCAAGGAGGATCTTTTAGTATTTCCAACTTG
  GGGATGTTTGGCATCGATGAGTTCGCTGCAGTGATTAACCCGCCTCAGGCCTGTATCTTGGCCGTTGGGA
  GATTCCGCCCCGTGCTGAAGCTGACGGAGGATGAAGAGGGGAACCCTCAACTGCAGCAGCACCAGCTTAT
  AACGGTCACAATGTCAAGTGACAGCCGAGTGGTTGATGATGAACTAGCTACCAGGTTTCTTGAAACTTTC
  AAAGCAAACCTAGAGAATCCCATGCGACTTGGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175094
• Insert Size: 1506 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175094.5, NP_780303.1
• RefSeq Size: 2527 bp
• RefSeq ORF: 1506 bp
• Locus ID: 27402
• UniProt ID: Q8BKZ9
• Cytogenetics: 2 E2
• Gene Summary: Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex (By similarity).[UniProtKB/Swiss-Prot Function]