Irf5 (NM_012057) Mouse Untagged Clone

CAT#: MC216989

Irf5 (untagged) - Mouse interferon regulatory factor 5 (Irf5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Irf5
• Synonyms: AW491843; mirf5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216989 representing NM_012057
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCACTCAGCCCCAGGGATTCCCCCACCACCCCGCCGTGTGAGGCTGAAGCCCTGGTTGGTGGCCC
  AGGTGAACAGCTGCCAGTACCCAGGGCTTCAGTGGGTCAACGGGGAAAAGAAACTCTTCTATATACCCTG
  GCGCCATGCCACGAGGCATGGTCCCAGCCAGGATGGGGACAACACCATCTTCAAGGCCTGGGCTAAAGAG
  ACAGGGAAGTACACTGAAGGGGTGGATGAGGCTGACCCAGCCAAGTGGAAGGCCAACCTGCGCTGTGCCC
  TTAACAAAAGCCGTGACTTCCAGCTGTTCTATGATGGCCCTCGGGACATGCCACCTCAGCCGTACAAGAT
  CTACGAGGTCTGCTCCAACGGCCCTGCTCCCACAGAGAGCCAACCCACTGATGATTACGTTCTGGGAGAA
  GAGGAGGAGGAGGAAGAGGAAGAGCTCCAGAGAATGCTACCAGGCCTGAGCATCACAGAGCCTGCGCTAC
  CTGGGCCTCCCAACGCACCCTATTCCTTACCCAAAGAAGACACCAAGTGGCCACCTGCTCTCCAGCCACC
  TGTAGGGCTGGGTCCCCCTGTCCCAGACCCAAATCTCCTGGCCCCTCCCTCTGGAAATCCTGCTGGCTTC
  AGGCAGCTTCTCCCTGAGGTCCTGGAGCCTGGACCTCTGGCTTCCAGCCAGCCCCCTACAGAACCACTCT
  TGCCTGACCTGCTGATCAGCCCCCACATGTTGCCTTTGACGGACCTAGAGATCAAGTTCCAGTACCGGGG
  ACGCGCACCCCGGACCCTCACCATCAGCAACCCACAAGGCTGCAGGCTCTTCTACAGCCAGCTAGAGGCT
  ACCCAGGAGCAAGTGGAACTCTTTGGCCCTGTGACCCTGGAGCAAGTGCGCTTCCCTAGCCCAGAGGACA
  TCCCCAGTGACAAGCAGCGTTTCTATACGAACCAGCTGCTAGATGTCCTGGACCGTGGGCTCATCCTGCA
  GCTGCAGGGCCAGGACCTGTACGCCATCCGTCTGTGCCAGTGTAAGGTGTTCTGGAGTGGGCCCTGCGCC
  TTGGCCCATGGCTCCTGCCCCAACCCCATTCAGCGGGAAGTCAAGACGAAGCTCTTTAGCCTAGAGCAGT
  TTCTCAATGAGCTCATCCTGTTCCAGAAGGGCCAGACTAATACCCCACCACCTTTTGAGATCTTCTTTTG
  CTTTGGAGAAGAATGGCCTGATGTCAAACCCCGAGAGAAGAAGCTCATTACTGTACAGGTGGTACCTGTT
  GCAGCCCGGTTGCTGCTGGAGATGTTCTCAGGGGAGCTTTCTTGGTCGGCAGACAGCATCCGACTGCAGA
  TCTCAAACCCGGATCTCAAAGACCACATGGTAGAGCAGTTTAAAGAGCTTCATCACCTCTGGCAGTCCCA
  GCAGCAATTGCAGCCCATGGTCCAGGCCCCTCCTGTGGCAGGCCTCGATGCAAGCCAGGGGCCCTGGCCC
  ATGCACCCAGTTGGCATGCAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_012057
• Insert Size: 1494 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012057.4, NP_036187.1
• RefSeq Size: 2206 bp
• RefSeq ORF: 1494 bp
• Locus ID: 27056
• UniProt ID: P56477
• Cytogenetics: 6 12.36 cM
• Gene Summary: Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains a different 5' end, which results in the use of an upstream start codon, compared to variant 1. The resulting protein (isoform 2) has a shorter and different N-terminus when it is compared to isoform 1.