Zfp207 (NM_001130169) Mouse Untagged Clone

CAT#: MC216979

Zfp207 (untagged) - Mouse zinc finger protein 207 (Zfp207), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zfp207
• Synonyms: 8430401D15Rik; BuGZ; Zep; Znf207
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216979 representing NM_001130169
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCGCAAGAAGAAGAAGCAGCTCAAGCCGTGGTGCTGGTATTGTAACAGAGATTTTGATGATGAGA
  AGATCCTTATACAGCACCAAAAAGCAAAGCATTTTAAATGCCATATTTGTCATAAGAAATTATACACAGG
  ACCTGGTTTAGCAATTCATTGCATGCAGGTCCATAAAGAAACCATAGATGCAGTACCAAATGCAATACCT
  GGGAGAACAGACATAGAGTTGGAAATATATGGCATGGAAGGTATTCCAGAGAAAGATATGGATGAAAGAC
  GGCGACTTCTTGAACAGAAAACACAAGAGAGTCAGAAAAAGAAACAACAAGATGATTCTGATGAATATGA
  TGATGATGAATCTGCAGCCTCAACTTCCTTTCAGCCACAGCCTGTTCAACCTCAGCAAGGTTATATCCCA
  CCAATGGCTCAGCCAGGACTGCCTCCAGTTCCAGGGGCACCAGGAATGCCTCCAGGCATACCTCCATTGA
  TGCCAGGTGTTCCTCCCCTGATGCCAGGCATGCCTCCAGTGATGCCAGGAATGCCGCCTGGATTGCATCA
  TCAGAGAAAATACACCCAGTCATTTTGCGGTGAAAACATAATGATGCCAATGGGTGGAATGATGCCACCT
  GGACCTGGAATACCACCTCTGATGCCAGGTATGCCACCAGGTATGCCCCCACCTGTTCCACGTCCTGGAA
  TTCCTCCAATGACTCAAGCACAGGCTGTTTCAGCACCAGGTATTCTTAATAGACCACCTGCACCAACAGC
  AGCAGTACCTGCTCCACAGCCTCCAGTTACTAAGCCTCTTTTCCCCAGTGCTGGACAGATGGGGACACCT
  GTAACAAGCTCAAGTACAGCTTCATCCAATTCAGAAAGTCTGTCTGCATCTTCTAAAGCTCTGTTTCCTA
  GCACAGCACAAGCTCAGGCAGCTGTCCAAGGACCTGTTGGTACAGATTTTAAGCCCTTAAATAGTACTCC
  TGCAGCAACAACTACAGAACCCCCAAAGCCTACATTCCCTGCTTATACACAGTCTACAGCGTCAACCACT
  AGTACAACAAACAGTACTGCAGCAAAGCCAGCAGCTTCAATAACAAGTAAGCCTGCTACACTCACAACCA
  CCAGTGCAACCAGTAAGTTGATCCATCCAGATGAGGATATATCACTGGAAGAAAGAAGGGCACAGTTACC
  TAAATATCAGAGAAATCTTCCTCGACCAGGACAAACTCCAATTGGTAATCCACCAGTTGGACCAATTGGG
  GGTATGATGCCACCACAGCCAGGCCTGCCACAGCAGCAGGCAATGCGACCTCCAATGCCACCTCATGGTC
  AGTATGGTGGTCATCATCAAGGCATGCCAGGTTATCTTCCTGGCGCTATGCCACCGTATGGACAGGGACC
  ACCAATGGTGCCCCCTTACCAAGGTGGGCCTCCTCGACCTCCAATGGGAATGAGACCTCCTGTAATGTCG
  CAAGGTGGCCGTTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001130169
• Insert Size: 1488 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130169.1, NP_001123641.1
• RefSeq Size: 2376 bp
• RefSeq ORF: 1488 bp
• Locus ID: 22680
• UniProt ID: Q9JMD0
• Cytogenetics: 11 B5
• Gene Summary: Kinetochore- and microtubule-binding protein that plays a key role in spindle assembly. ZNF207/BuGZ is mainly composed of disordered low-complexity regions and undergoes phase transition or coacervation to form temperature-dependent liquid droplets. Coacervation promotes microtubule bundling and concentrates tubulin, promoting microtubule polymerization and assembly of spindle and spindle matrix by concentrating its building blocks (PubMed:26388440). Also acts as a regulator of mitotic chromosome alignment by mediating the stability and kinetochore loading of BUB3. Mechanisms by which BUB3 is protected are unclear: according to a first report, ZNF207/BuGZ may act by blocking ubiquitination and proteasomal degradation of BUB3. According to another report, the stabilization is independent of the proteasome (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).