Xiap (NM_009688) Mouse Untagged Clone

CAT#: MC216947

Xiap (untagged) - Mouse X-linked inhibitor of apoptosis (Xiap), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Xiap
• Synonyms: 1110015C02Rik; A; Aipa; Api3; Bir; Birc4; I; IAP3; IL; ILP-1; MIHA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216947 representing NM_009688
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTTTAACAGTTTTGAAGGAACTAGAACTTTTGTACTTGCAGACACCAATAAGGATGAAGAATTTG
  TAGAAGAGTTTAATAGATTAAAAACATTTGCTAACTTCCCAAGTAGTAGTCCTGTTTCAGCATCAACATT
  GGCGCGAGCTGGGTTTCTTTATACCGGTGAAGGAGACACCGTGCAATGTTTCAGTTGTCATGCGGCAATA
  GATAGATGGCAGTATGGAGACTCAGCTGTTGGAAGACACAGGAGAATATCCCCAAATTGCAGATTTATCA
  ATGGTTTTTATTTTGAAAATGGTGCTGCACAGTCTACAAATCCTGGTATCCAAAATGGCCAGTACAAATC
  TGAAAACTGTGTGGGAAATAGAAATCCTTTTGCCCCTGACAGGCCACCTGAGACTCATGCTGATTATCTC
  TTGAGAACTGGACAGGTTGTAGATATTTCAGACACCATATACCCGAGGAACCCTGCCATGTGTAGTGAAG
  AAGCCAGATTGAAGTCATTTCAGAACTGGCCGGACTATGCTCATTTAACCCCCAGAGAGTTAGCTAGTGC
  TGGCCTCTACTACACAGGGGCTGATGATCAAGTGCAATGCTTTTGTTGTGGGGGAAAACTGAAAAATTGG
  GAACCCTGTGATCGTGCCTGGTCAGAACACAGGAGACACTTTCCCAATTGCTTTTTTGTTTTGGGCCGGA
  ACGTTAATGTTCGAAGTGAATCTGGTGTGAGTTCTGATAGGAATTTCCCAAATTCAACAAACTCTCCAAG
  AAATCCAGCCATGGCAGAATATGAAGCACGGATCGTTACTTTTGGAACATGGACATCCTCAGTTAACAAG
  GAGCAGCTTGCAAGAGCTGGATTTTATGCTTTAGGTGAAGGCGATAAAGTGAAGTGCTTTCACTGTGGAG
  GAGGGCTCACGGATTGGAAGCCAAGTGAAGACCCTTGGGAACAGCATGCGAAGTGGTACCCAGGGTGCAA
  ATACCTATTGGATGAGAAGGGGCAAGAATATATAAATAATATTCATTTAACCCATTCACTTGAGGAATCT
  TTGGGAAGAACTGCTGAAAAAACACCATCGCTAACTAAAAAAATCGATGATACCATCTTCCAGAATCCTA
  TGGTGCAAGAAGCTATACGAATGGGATTTAGCTTCAAGGACATTAAGAAAACAATGGAAGAAAAAATCCA
  AACATCCGGGAGCAGCTATCTATCACTTGAGGTCCTGATTGCAGATCTTGTGAGTGCTCAGAAAGATAAT
  ACGGAGGATGAGTCAAGTCAAACTTCATTGCAGAAAGACATTAGTACTGAAGAGCAGCTAAGGCGCCTAC
  AAGAGGAGAAGCTTTGCAAAATCTGTATGGATAGAAATATTGCTATCGTTTTTGTTCCTTGTGGACATCT
  GGTCACTTGTAAACAGTGTGCAGAAGCAGTTGACAAATGTCCCATGTGCTACACCGTCATTACGTTCAAG
  CAAAAAATTTTTATGTCTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009688
• Insert Size: 1491 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009688.3, NP_033818.2
• RefSeq Size: 6566 bp
• RefSeq ORF: 1491 bp
• Locus ID: 11798
• UniProt ID: Q60989
• Cytogenetics: X A4
• Gene Summary: The protein encoded by this gene is a member of the inhibitor of apoptosis (IAP) family of proteins. While first identified for its role in blocking apoptosis, this protein modulates many other signaling processes including nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) pathways and inflammatory responses. This protein blocks apoptosis by binding and inhibiting target caspases after they have been activated. Binding occurs to some, but not all, caspases. This protein has several conserved regions, including baculoviral IAP repeat (BIR) motifs and a RING finger E3 ligase domain. In humans, mutations in this gene are linked to immunodeficiency in X-linked lymphoproliferative syndrome type-2 (XLP-2). A pseudogene of this gene is found on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.