Fbxl7 (NM_176959) Mouse Untagged Clone

CAT#: MC216923

Fbxl7 (untagged) - Mouse F-box and leucine-rich repeat protein 7 (Fbxl7), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fbxl7
• Synonyms: AL023057; D230018M15Rik; Fbl6; FBL7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216923 representing NM_176959
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCGAACAATGGCAAACAGTACGGCAGTGAGGGCAAAGGCAGCTCAAGCGTCTCATCCGACGTGA
  GTTCAAGCACAGATCATACACCAACCAAAGCCCAGAGGAATGTGGCCACCAGCGAAGATTCTGACTTGAG
  CATGCGCACACTGAGCACGCCCAGCCCAGCCTTGATATGCCCACCGACTTTGCCAGGATTTCAGAATGGA
  AGGGGTTCGTCCACATCTTCATCCTCCATCACCGGGGAGACAGTGGCTATGGTTCACTCCCCGCCCCCGA
  CCCGCCTCACTCACCCACTCATTCGGCTCGCCTCCAGACCCCAGAAAGAGCAAGCCAGCATAGACAGGCT
  CCCGGACCACTCCATGGTGCAGATCTTCTCCTTCCTGCCCACCAACCAGCTATGTCGCTGTGCACGTGTG
  TGCCGCCGCTGGTACAACCTGGCCTGGGACCCGCGCCTCTGGAGGACTATCCGTCTCACTGGAGAGACCA
  TCAATGTGGACCGTGCCCTGAAAGTACTGACCCGCAGGCTTTGCCAGGACACCCCCAATGTGTGTCTCAT
  GCTGGAGACTGTCATTGTCAGTGGCTGCCGGCGGCTCACAGACCGGGGGCTTTATACCATTGCACAGTGC
  TGTCCAGAACTGAGGCGCCTGGAAGTCTCAGGATGTTACAATATCTCGAACGAGGCTGTCTTTGATGTGG
  TGTCACTCTGTCCCAACCTGGAGCATCTGGATGTGTCAGGGTGCTCCAAAGTGACCTGCATCAGCTTGAC
  CCGGGAGGCCTCCATTAAACTGTCCCCCTTACATGGCAAACAGATTTCCATCCGATACCTTGATATGACG
  GACTGCTTCGTGCTGGAGGACGAAGGCTTACACACCATCGCAGCTCACTGCACGCAGCTCACTCACCTCT
  ATCTGCGCCGCTGCGTCCGCCTCACAGATGAGGGTCTCCGCTACCTGGTGATCTACTGCACTTCCATCAA
  GGAGCTGAGTGTCAGCGACTGCCGCTTTGTCAGTGACTTTGGCCTGCGGGAGATTGCTAAACTGGAGTCC
  CGCTTGAGGTACCTCAGCATCGCCCACTGCGGCCGCATCACGGATGTGGGCATTCGTTATGTGGCTAAAT
  ACTGCAGCAAATTACGCTACCTCAATGCGAGGGGCTGCGAGGGCATCACGGACCATGGTGTGGAGTACCT
  CGCCAAGAACTGTACAAAACTTAAGTCGCTGGACATTGGTAAATGTCCCCTGGTCTCCGACACGGGCCTG
  GAATCCCTGGCTTTGAACTGCTTCAATCTCAAGAGGCTAAGCCTCAAGTCCTGTGAGAGCATCACCGGCC
  AGGGCCTGCAGATCGTGGCTGCCAACTGCTTCGACCTGCAGATGTTAAATGTCCAGGACTGTGAAGTTTC
  CGTGGAGGCCCTGCGGTTTGTGAAGCGCCATTGCAAGCGCTGTGTCATTGAGCATACCAACCCCGCTTTC
  TTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_176959
• Insert Size: 1476 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176959.3, NP_795933.2
• RefSeq Size: 4639 bp
• RefSeq ORF: 1476 bp
• Locus ID: 448987
• UniProt ID: Q5BJ29
• Cytogenetics: 15 B1
• Gene Summary: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of AURKA during mitosis, causing mitotic arrest.[UniProtKB/Swiss-Prot Function]