Tbx1 (NM_011532) Mouse Untagged Clone

CAT#: MC216816

Tbx1 (untagged) - Mouse T-box 1 (Tbx1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tbx1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_011532, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATCTCCGCCGTGTCTAGTCCGTGGCTCACGCAGCTCTCGCACTTCTGCGACGTTGCAGCCTTCGCAG
  CCAGCAGTCTGAGCGGCCTGGGATCCCCGTCGCCTGGCGCCGACCCGTTCGGCCCTCGCGAGCCGCCGCC
  ACCGCGCTACGATCCGTGCGCTGCAGTCCCCGGTGCCCCGGGCCCGCCGCCGCCGCGCGCCTATCCTTTC
  GCGCCCGCCCCCGGGGCGGCTGGCAGCTCGGCGGCGGAGTCCGAGGGTCCGGGGGCTAGCCGCGCGGCTG
  CGGTCAAGGCTCCGGTGAAGAAGAACCCGAAGGTGGCCAGCGTGAGCGTGCAGCTGGAGATGAAGGCGCT
  GTGGGACGAGTTCAATCAGCTGGGCACCGAGATGATCGTCACCAAGGCAGGCAGACGAATGTTCCCCACG
  TTCCAAGTGAAGCTTTTTGGAATGGATCCCATGGCCGACTACATGCTGCTCATGGACTTTGTGCCCGTAG
  ATGACAAGCGCTACCGGTATGCTTTCCATAGCTCCTCCTGGCTGGTGGCCGGCAAGGCAGATCCTGCTAC
  ACCTGGCCGAGTACACTACCACCCGGACTCGCCGGCTAAGGGCGCACAGTGGATGAAACAGATTGTGTCT
  TTCGACAAGCTGAAACTGACCAATAACCTGCTGGATGACAATGGCCATATTATTCTCAACTCCATGCACA
  GATATCAGCCCCGATTCCATGTTGTCTATGTGGACCCTCGAAAAGACAGTGAGAAATATGCAGAGGAGAA
  CTTCAAAACTTTTGTGTTTGAGGAGACACGCTTCACTGCAGTCACTGCCTACCAGAATCACCGGATCACG
  CAGCTTAAGATTGCCAGCAACCCCTTCGCCAAAGGCTTCCGGGATTGCGACCCGGAGGACTGGCCCCGGA
  ACCACCGGCCCGGAGCGCTGCCGCTCGTGAGTGCCTTTGCTCGCTCTCGGAATCCCGTGGCTTCCCCCAC
  GCAGCCCAATGGCTCAGACAAAGACGCTGCAGAAGCCCGGCGCGAGTTCGACCGTGACTCCGGACCCGCA
  GCGCTCGGCGACGCTACGCACCCGCCGCAGCTGCTGGCGCGCGTGCTGAGCCCCGCACTGCCCGGGCCTG
  GCGGCCTCGTCCCGCTACCCGGCGGATCCGGAGGCCGCCACAGTCCCCCGCACGCCGATCTGCGCCTGGA
  GGCGCCGGGCGCGTCCGAGCCGCTGCACCACCATCCCTACAAGTACCCGGCCGCCGCCTACGACCACTAC
  CTCGGGGCCAAGAGCCGGCCGGCGCCCTACCCGCTGCCAGGCCTGCGCGGCCACGGCTACCACCCGCACG
  CGCACCCGCACGCGCACCCGCACCATCACCACCACCCCGCGGTGAACCCGGCCGCCGCCGCCGCTGCTGC
  CGCAGCAGCCAACGTGTACTCGTCGGCGGCCGCGCCGCCCGGTGCCTACGACTACTGCCCCAGATAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_011532
• Insert Size: 1467 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC139474, AAI39475
• RefSeq Size: 1686 bp
• RefSeq ORF: 1467 bp
• Locus ID: 21380
• UniProt ID: P70323
• Cytogenetics: 16
• Gene Summary: Probable transcriptional regulator involved in developmental processes. Is required for normal development of the pharyngeal arch arteries.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1).