Trim72 (NM_001079932) Mouse Untagged Clone

CAT#: MC216581

Trim72 (untagged) - Mouse tripartite motif-containing 72 (Trim72), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim72
• Synonyms: BC067209; MG53
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216581 representing NM_001079932
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCTGCACCCGGCCTTCTGCGTCAGGAACTGTCCTGCCCACTGTGCTTGCAGCTGTTCGATGCGC
  CAGTGACGGCTGAGTGTGGCCACAGTTTCTGCCGTGCCTGCCTGATCCGGGTGGCAGGGGAGCCTGCTGC
  GGACGGCACAGTTGCCTGTCCCTGTTGTCAGGCACCTACACGGCCGCAGGCTCTAAGCACTAACCTCCAG
  TTGTCACGCCTTGTGGAGGGTTTGGCGCAAGTGCCCCAAGGCCACTGCGAGGAACACCTGGATCCACTGA
  GCATCTACTGCGAGCAGGACCGCACACTTGTGTGTGGTGTGTGTGCCTCGCTCGGTTCTCACCGTGGTCA
  TCGTCTCCTGCCTGCCGCTGAAGCCCAAGCACGCCTCAAGACACAGCTTCCACAGCAGAAGATGCAGCTG
  CAGGAGGCATGCATGCGCAAGGAGAAGACTGTAGCGGTGCTGGAGCATCAGCTGGTGGAGGTGGAGGAGA
  CAGTGCGCCAGTTCCGGGGAGCTGTCGGGGAGCAGCTGGGGAAGATGCGGATGTTCCTGGCTGCCCTAGA
  AAGTTCTCTGGACCGTGAAGCAGAAAGGGTTCGGGGTGATGCTGGGGTTGCCTTGCGTCGGGAGCTGTCA
  AGCCTGAACTCTTACCTAGAGCAACTGAGGCAGATGGAGAAGGTGCTGGAGGAGGTGGCTGACAAGCCAC
  AGACAGAATTCCTCATGAAATTCTGCCTGGTAACCAGCAGGCTGCAGAAGATCCTGTCAGAGTCACCACC
  ACCGGCAAGGCTAGATATCCAGCTGCCTGTCATCTCAGATGACTTCAAATTCCAGGTGTGGAAGAAGATG
  TTCCGGGCTCTGATGCCAGCGCTGGAGGAACTGACTTTTGACCCCAGCTCTGCGCACCCGAGCCTGGTGG
  TGTCCTCCTCTGGTCGCCGAGTGGAGTGCTCAGACCAGAAGGCGCCGCCAGCGGGAGAAGACACGCGTCA
  GTTCGACAAGGCAGTAGCGGTGGTGGCGCAGCAGCTGCTGTCACAGGGCGAGCACTATTGGGAGGTGGAG
  GTGGGCGACAAACCACGCTGGGCCCTGGGAGTGATGGCGGCTGACGCTTCCCGCCGTGGCCGGCTGCACG
  CGGTGCCCTCACAGGGGCTGTGGCTGCTGGGTCTGCGCGATGGCAAGATCCTGGAGGCGCACGTGGAGGC
  CAAGGAGCCGCGGGCACTGCGCACCCCAGAGAGGCCTCCGGCGCGCATTGGCCTCTACCTAAGCTTCGCA
  GATGGCGTCCTGGCTTTCTATGATGCGAGCAACCCCGACGTACTTACGCCAATCTTTTCTTTCCACGAGC
  GTCTGCCCGGGCCGGTGTACCCCATCTTTGACGTGTGCTGGCACGACAAGGGCAAGAATGCCCAGCCCCT
  GCTGCTTGTGGGGCCGGAGCAGGAACAGGCCTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001079932
• Insert Size: 1434 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079932.3, NP_001073401.1
• RefSeq Size: 2472 bp
• RefSeq ORF: 1434 bp
• Locus ID: 434246
• UniProt ID: Q1XH17
• Cytogenetics: 7 F3
• Gene Summary: Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.[UniProtKB/Swiss-Prot Function]