Cpe (NM_013494) Mouse Untagged Clone

CAT#: MC216440

Cpe (untagged) - Mouse carboxypeptidase E (Cpe), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cpe
• Synonyms: CP; CPH; Cph-; Cph-1; Cph1; fat; NF-a; NF-alpha1; R74677
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216440 representing NM_013494
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGGCGCGGAGGACGGGTGCTGCTGGCGCTGTGTGCCGCGCTGGTGGCCGGCGGGTGGCTGCTGA
  CGGCTGAAGCCCAGGAGCCCGGGGCGCCAGCGGCTGGCATGAGGCGCCGCCGGCGGCTCCAGCAAGAGGA
  CGGCATCTCCTTCGAGTACCACCGCTATCCAGAGCTGCGCGAGGCGCTGGTGTCCGTATGGCTGCAGTGC
  ACCGCCATCAGCAGAATCTACACAGTGGGGCGCAGCTTCGAGGGCCGGGAGCTCCTGGTCATCGAGCTGT
  CTGACAACCCCGGGGTCCATGAGCCGGGTGAACCTGAATTTAAATACATTGGGAACATGCATGGTAATGA
  GGCGGTTGGACGGGAACTGCTTATTTTCTTGGCCCAGTACCTGTGTAACGAGTACCAGAAAGGCAATGAG
  ACAATTGTCAACCTGATCCACAGCACCCGAATTCATATCATGCCCTCCTTGAACCCCGACGGCTTTGAGA
  AAGCCGCATCGCAGCCCGGCGAGCTGAAGGACTGGTTTGTGGGCCGCAGCAACGCCCAGGGAATAGATCT
  GAACCGTAACTTCCCAGACCTGGACAGGATCGTGTATGTTAATGAGAAAGAAGGCGGTCCTAACAATCAC
  CTGCTGAAGAATCTGAAGAAAATTGTGGACCAAAATTCAAAGCTTGCCCCCGAGACCAAGGCTGTCATTC
  ACTGGATCATGGACATTCCATTTGTGCTTTCTGCCAATCTGCACGGAGGAGACCTTGTGGCTAATTACCC
  ATATGATGAGACACGGAGCGGTACTGCTCACGAATACAGTTCCTGCCCTGATGACGCAATTTTCCAAAGC
  TTGGCTCGCGCGTACTCTTCTTTCAACCCAGTCATGTCTGACCCCAATCGACCTCCCTGTCGCAAGAATG
  ACGATGACAGCAGCTTTGTAGATGGAACGACCAATGGTGGTGCATGGTACAGCGTCCCCGGTGGAATGCA
  AGACTTCAATTACCTGAGCAGCAACTGCTTCGAGATCACTGTGGAGCTTAGCTGTGAGAAGTTCCCACCG
  GAAGAGACTCTCAAAAGCTACTGGGAAGATAACAAAAACTCCCTCATCAGCTACCTGGAGCAGATACACC
  GAGGTGTTAAAGGGTTTGTCCGTGACCTTCAGGGTAACCCGATTGCCAACGCAACCATCTCTGTGGACGG
  GATAGACCATGATGTCACCTCGGCTAAGGATGGGGATTACTGGCGATTGCTTGCTCCTGGAAACTATAAA
  CTTACAGCCTCCGCTCCTGGCTACCTGGCAATCACAAAGAAAGTGGCAGTTCCTTTTAGCCCTGCTGTTG
  GGGTGGACTTTGAGCTTGAGTCTTTCTCTGAAAGGAAGGAGGAGGAGAAGGAAGAATTGATGGAGTGGTG
  GAAAATGATGTCAGAAACTTTGAATTTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013494
• Insert Size: 1431 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013494.4, NP_038522.2
• RefSeq Size: 2183 bp
• RefSeq ORF: 1431 bp
• Locus ID: 12876
• UniProt ID: Q00493
• Cytogenetics: 8 32.3 cM
• Gene Summary: This gene encodes carboxypeptidase E, a prohormone-processing exopeptidase found in secretory granules of endocrine and neuroendocrine cells. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional enzyme that cleaves the C-terminal basic residues of protein substrates. A missense mutation in this gene is responsible for the obesity phenotype in a mouse model known as the "fat mouse." Mice lacking the functional product of this gene exhibit impaired processing of multiple peptide hormones such as proinsulin, prodynorphin, proneurotensin, promelanin-concentrating hormone and pro-opiomelanocortin. [provided by RefSeq, Jan 2016]