Ppm1h (NM_176919) Mouse Untagged Clone

CAT#: MC216386

Ppm1h (untagged) - Mouse protein phosphatase 1H (PP2C domain containing) (Ppm1h), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ppm1h
• Synonyms: A430075L18Rik; ARHCL1; C030002B11Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216386 representing NM_176919
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCACTCGAGTGAAATCTGCCGTGGCCAATTTCATGGGCGGCATCATGGCTGGCAGCTCCGGCTCCG
  AGCACGGCGGGAGCGGCTGCGGAGGCTCGGACCTGCCCCTGCGCTTCCCGTACGGGCGGCCGGAGTTCCT
  CGGGCTGTCTCAGGATGAGGTGGAGTGCAGCGCAGACCACATCGCCCGCCCCATCCTCATCCTCAAGGAG
  ACCCGGCGGCTGCCCTGGGCCACTGGCTACGCAGAGGTTATTAATGCTGGGAAGAGCACACACAATGAGG
  ACCAAGCCAGCTGCGAGGTGCTCACAGTGAAGAAGAAAGCCGGAACCATTACTTCCACCCCGAACAGAAA
  CTCCAAGAGAAGGTCATCACTTCCCAACGGGGAAGGCCTGCAGTTAAAGGAGAACTCTGAGTCCGAAGGC
  ATCTCCTGCCACTACTGGTCCCTGTTTGACGGTCACGCAGGCTCGGGGGCTGCCGTGGTGGCATCGCGCC
  TGCTGCAGCACCACATCACGCAACAGCTGCAGGACATCGTGGAGATCCTGAAGAACTCGGCCATCCTTCC
  CCCGACCTGCCTGGGGGAGGAGCCAGAGAGCACGCCAGCCCACGGCAGGACCCTAACCCGCGCGGCCTCA
  CTGCGTGGAGGTGTGGGTGCCCCGGGCTCCCCCAGTACGCCACCCACGCGCTTCTTCACTGAGAAGAAGA
  TCCCGCACGAGTGTCTGGTCATCGGGGCCCTGGAGAGCGCTTTCAAGGAAATGGACCTTCAAATCGAACG
  CGAGAGGAGTGCGTATAATATATCTGGCGGCTGCACGGCCCTCATCGTGGTTTGCCTCTTGGGGAAGCTC
  TATGTGGCGAATGCTGGCGACAGCAGGGCCATAATCATCAGAAATGGAGAAATCATCCCCATGTCTTCCG
  AATTCACCCCTGAGACTGAGCGCCAGAGACTTCAGTACCTGGCGTTCATGCAGCCCCACTTGCTGGGAAA
  TGAGTTCACACATTTGGAGTTTCCAAGGAGAGTGCAGAGGAAGGAACTCGGGAAGAAGATGCTGTACCGG
  GACTTTAATATGACAGGCTGGGCATACAAAACCATTGAGGATGATGACTTGAAGTTCCCCCTTATATATG
  GAGAAGGCAAGAAGGCCCGAGTAATGGCAACTATTGGAGTGACGAGGGGACTTGGGGACCATGACTTAAA
  GGTTCATGACTCCAACATCTACATAAAACCATTCCTGTCTTCAGCTCCAGAGGTCAGAGTCTACGATCTC
  TCCAGATATGAGCACGGAGCAGATGACGTGCTGATCCTGGCTACTGATGGACTCTGGGATGTCTTATCCA
  ACGAAGAAGTAGCGGAAGCAATCACTCAGTTTCTTCCTAACTGTGATCCAGACGACCCTCACAGGTTTGT
  GCCTTTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_176919
• Insert Size: 1410 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176919.4, NP_795893.2
• RefSeq Size: 2947 bp
• RefSeq ORF: 1410 bp
• Locus ID: 319468
• UniProt ID: Q3UYC0
• Cytogenetics: 10 D2
• Gene Summary: Dephosphorylates CDKN1B at 'Thr-187', thus removing a signal for proteasomal degradation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks the 3' terminal exon and its transcription extends past a splice site that is used in variant 1, resulting in a novel 3' coding region and 3' UTR compared to variant 1. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1.