Samm50 (NM_178614) Mouse Untagged Clone

CAT#: MC216323

Samm50 (untagged) - Mouse sorting and assembly machinery component 50 homolog (S. cerevisiae) (Samm50), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Samm50
• Synonyms: 1110030L07Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216323 representing NM_178614
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACAGTGCACGCCCGGAGTCTGGAGCCCCTCCCGTCGAGTGGAACTGACTTTGGAGCACTGGGGG
  AGGAAGCCGAGTTTGTGGAAGTTGAGCCGGAAGCGAAACAGGAAATCCTGGAAAACAAAGATGTGGTGGT
  TCAGCATGTTCATTTTGATGGACTTGGGCGGACTAAGGATGACATCATCATTTGTGAAATCGGAGAGGTC
  TTTAAGGCTAAAAACCTCATTGAGGTAATGCGGCGATCTCATGAAGCCCGGGAAAAACTGCTTCGCCTAG
  GAATTTTTAGACAAGTGGATGTTTTGATCGATACATGTCATGGTGAAGATGCCCTGCCCAATGGGTTAGA
  TGTCACCTTTGAAGTGACAGAGCTGAGGAGACTGACGGGCAGTTACAACACCATGGTTGGAAACAACGAA
  GGCAGTATGGTACTCGGCCTCAAACTCCCCAACCTTCTGGGACGAGCAGAAAAAGTCACTTTCCAGTTTT
  CTTATGGAACCAAAGAAACTTCCTACGGCCTGTCCTTCTTCAAGCCACAGCCTGGAAACTTCGAGAGAAA
  TTTCTCCGTAAACTTATATAAAGTTACTGGGCAGTTCCCGTGGAGCTCACTTCGGGAGACAGACAGAGGA
  GTGTCTGCAGAGTACAGTTTTCCCCTGTGGAAGACCAGTCACACTGTCAAGTGGGAGGGTGTGTGGCGGG
  AGCTGGGCTGCCTCTCGAGGACTGCGTCGTTCGCTGTGCGGAAGGAAAGTGGACACTCACTGAAGTCGTC
  TCTCTCGCATGCCATGGTCATCGACTCTCGAAATTCATCTATCTTGCCAAGAAGAGGGGCCTTGTTCAAA
  GTCAACCAGGAGCTGGCAGGCTACACTGGAGGAGATGTGAGCTTCATCAAGGAAGACTTTGAGCTTCAGC
  TGAATAAGCCGCTCGCCTTGGACTCGGTATTCTCCACGTCTCTCTGGGGTGGAATGCTGGTGCCCATCGG
  TGACAAGCCATCCAGCATTGCTGACAGGTTTTACCTGGGAGGCCCCACGAGTGTCCGAGGATTTAGCATG
  CACAGCATTGGACCCCAGAGTGAAGGAGATTACCTGGGCGGCGAGGCCTACTGGGCTGGGGGCCTGCACC
  TCTACACCCCACTGCCCTTCCGGCCAGGCCAGGGTGGCTTCGGAGAGCTTTTCAGAACTCACTTTTTCCT
  CAATGCGGGCAACCTGTGCAACCTCAACTATGGTGAGGGCCCCAAAGCCCATATCCGGAAGCTAGCTGAG
  TGCATCCGCTGGTCCTATGGAGCAGGCGTCGTCCTCCGACTTGGCAACATCGCTCGGCTGGAGCTGAACT
  ACTGCATTCCTATGGGCGTGCAGGGGGGCGACAGGATTTGTGATGGTGTCCAGTTTGGAGCTGGGATTCG
  GTTCCTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178614
• Insert Size: 1410 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178614.4, NP_848729.1
• RefSeq Size: 1673 bp
• RefSeq ORF: 1410 bp
• Locus ID: 68653
• UniProt ID: Q8BGH2
• Cytogenetics: 15 E2
• Gene Summary: Plays a crucial role in the maintenance of the structure of mitochondrial cristae and the proper assembly of the mitochondrial respiratory chain complexes. Required for the assembly of TOMM40 into the TOM complex.[UniProtKB/Swiss-Prot Function]