Samm50 (NM_178614) Mouse Tagged ORF Clone

CAT#: MG222025

Samm50 (tGFP-tagged) - Mouse sorting and assembly machinery component 50 homolog (S. cerevisiae) (Samm50), (10ug)

Product Images

Circular map for MG222025

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Samm50
• Synonyms: 1110030L07Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222025 representing NM_178614
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACAGTGCACGCCCGGAGTCTGGAGCCCCTCCCGTCGAGTGGAACTGACTTTGGAGCACTGGGGG
  AGGAAGCCGAGTTTGTGGAAGTTGAGCCGGAAGCGAAACAGGAAATCCTGGAAAACAAAGATGTGGTGGT
  TCAGCATGTTCATTTTGATGGACTTGGGCGGACTAAGGATGACATCATCATTTGTGAAATCGGAGAGGTC
  TTTAAGGCTAAAAACCTCATTGAGGTAATGCGGCGATCTCATGAAGCCCGGGAAAAACTGCTTCGCCTAG
  GAATTTTTAGACAAGTGGATGTTTTGATCGATACATGTCATGGTGAAGATGCCCTGCCCAATGGGTTAGA
  TGTCACCTTTGAAGTGACAGAGCTGAGGAGACTGACGGGCAGTTACAACACCATGGTTGGAAACAACGAA
  GGCAGTATGGTACTCGGCCTCAAACTCCCCAACCTTCTGGGACGAGCAGAAAAAGTCACTTTCCAGTTTT
  CTTATGGAACCAAAGAAACTTCCTACGGCCTGTCCTTCTTCAAGCCACAGCCTGGAAACTTCGAGAGAAA
  TTTCTCCGTAAACTTATATAAAGTTACTGGGCAGTTCCCGTGGAGCTCACTTCGGGAGACAGACAGAGGA
  GTGTCTGCAGAGTACAGTTTTCCCCTGTGGAAGACCAGTCACACTGTCAAGTGGGAGGGTGTGTGGCGGG
  AGCTGGGCTGCCTCTCGAGGACTGCGTCGTTCGCTGTGCGGAAGGAAAGTGGACACTCACTGAAGTCGTC
  TCTCTCGCATGCCATGGTCATCGACTCTCGAAATTCATCTATCTTGCCAAGAAGAGGGGCCTTGTTCAAA
  GTCAACCAGGAGCTGGCAGGCTACACTGGAGGAGATGTGAGCTTCATCAAGGAAGACTTTGAGCTTCAGC
  TGAATAAGCCGCTCGCCTTGGACTCGGTATTCTCCACGTCTCTCTGGGGTGGAATGCTGGTGCCCATCGG
  TGACAAGCCATCCAGCATTGCTGACAGGTTTTACCTGGGAGGCCCCACGAGTGTCCGAGGATTTAGCATG
  CACAGCATTGGACCCCAGAGTGAAGGAGATTACCTGGGCGGCGAGGCCTACTGGGCTGGGGGCCTGCACC
  TCTACACCCCACTGCCCTTCCGGCCAGGCCAGGGTGGCTTCGGAGAGCTTTTCAGAACTCACTTTTTCCT
  CAATGCGGGCAACCTGTGCAACCTCAACTATGGTGAGGGCCCCAAAGCCCATATCCGGAAGCTAGCTGAG
  TGCATCCGCTGGTCCTATGGAGCAGGCGTCGTCCTCCGACTTGGCAACATCGCTCGGCTGGAGCTGAACT
  ACTGCATTCCTATGGGCGTGCAGGGGGGCGACAGGATTTGTGATGGTGTCCAGTTTGGAGCTGGGATTCG
  GTTCCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222025 representing NM_178614
  Red=Cloning site Green=Tags(s)
  
  MGTVHARSLEPLPSSGTDFGALGEEAEFVEVEPEAKQEILENKDVVVQHVHFDGLGRTKDDIIICEIGEV
  FKAKNLIEVMRRSHEAREKLLRLGIFRQVDVLIDTCHGEDALPNGLDVTFEVTELRRLTGSYNTMVGNNE
  GSMVLGLKLPNLLGRAEKVTFQFSYGTKETSYGLSFFKPQPGNFERNFSVNLYKVTGQFPWSSLRETDRG
  VSAEYSFPLWKTSHTVKWEGVWRELGCLSRTASFAVRKESGHSLKSSLSHAMVIDSRNSSILPRRGALFK
  VNQELAGYTGGDVSFIKEDFELQLNKPLALDSVFSTSLWGGMLVPIGDKPSSIADRFYLGGPTSVRGFSM
  HSIGPQSEGDYLGGEAYWAGGLHLYTPLPFRPGQGGFGELFRTHFFLNAGNLCNLNYGEGPKAHIRKLAE
  CIRWSYGAGVVLRLGNIARLELNYCIPMGVQGGDRICDGVQFGAGIRFL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178614
• ORF Size: 1407 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178614.5
• RefSeq Size: 1673 bp
• RefSeq ORF: 1410 bp
• Locus ID: 68653
• UniProt ID: Q8BGH2
• Cytogenetics: 15 E2
• Gene Summary: Plays a crucial role in the maintenance of the structure of mitochondrial cristae and the proper assembly of the mitochondrial respiratory chain complexes. Required for the assembly of TOMM40 into the TOM complex.[UniProtKB/Swiss-Prot Function]