Rgs7 (NM_011880) Mouse Untagged Clone

CAT#: MC216291

Rgs7 (untagged) - Mouse regulator of G protein signaling 7 (Rgs7), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rgs7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216291 representing NM_011880
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGGGAAATAATTATGGACAGACCAGCAACGGGGTGGCGGACGAATCACCCAACATGCTGGTGT
  ACAGAAAGATGGAAGATGTCATAGCACGTATGCAAGACGAGAAAAACGGAATTCCCATCCGTACCGTCAA
  AAGCTTCCTTTCCAAGATCCCCAGTGTGTTCTCCGGGTCAGACATTGTTCAATGGTTAATAAAGAACTTA
  ACTATAGAAGATCCAGTGGAGGCTCTCCATTTGGGAACGCTAATGGCTGCCCATGGCTACTTCTTTCCCA
  TCTCAGATCATGTCCTCACACTCAAGGATGACGGCACCTTCTACCGGTTTCAAACACCCTATTTTTGGCC
  GTCAAATTGTTGGGAGCCAGAAAACACAGACTATGCTGTTTACCTCTGCAAGAGAACAATGCAAAACAAG
  GCAAGGCTAGAGCTAGCAGATTATGAAGCCGAGAGCCTGGCCAGGCTGCAGAGAGCATTTGCCCGGAAGT
  GGGAGTTCATTTTTATGCAAGCAGAAGCACAAGCTAAAGTGGACAAGAAGAGAGACAAAATTGAAAGGAA
  GATCCTCGATAGTCAAGAGAGAGCATTCTGGGATGTCCACAGGCCTGTGCCTGGATGTGTAAATACTACA
  GAAGTGGACATTAAGAAGTCATCCCGGATGAGAAACCCACACAAAACACGAAAGTCTGTCTATGGTTTAC
  AAAATGACATCCGAAGTCACAGTCCCACCCACACACCTACACCAGAAACCAAGCCTCCTACAGAAGATGA
  GCTGCACCAACAGATAAAATACTGGCAAATACAGTTAGATAGACATCGGTTAAAAATGTCAAAAGTTGCT
  GATAGTCTACTAAGCTATACGGAACAGTATGTAGAATATGACCCGTTTCTTGTGCCGCCTGACCCTTCCA
  ATCCATGGCTCTCAGATGATACGACTTTCTGGGAACTTGAAGCAAGCAAAGAACCAAGTCAACAGAGGGT
  AAAACGATGGGGTTTTGGTATGGATGAGGCATTGAAAGACCCAGTTGGGCGAGAGCAGTTCCTTAAGTTT
  CTAGAATCAGAATTCAGCTCAGAGAACTTAAGGTTCTGGTTGGCAGTGGAGGACCTGAAGAGAAGGCCTA
  TCCGAGAGGTCCCCTCGAGAGTGCAGGAAATATGGCAAGAATTTCTGGCTCCTGGGGCCCCCAGTGCCAT
  TAACTTGGATTCTAAGAGTTATGACAAGACCACACAGAATGTGAAGGAACCAGGACGATACACATTTGAA
  GATGCTCAGGAGCATATTTACAAGCTGATGAAGAGCGACTCATACCCCCGCTTTATAAGATCTAGTGCCT
  ACCAGGAACTTCTACAGGCAAAGAGAAAGGGAAAAACTCTCACATCCAAGAGGTTAACAAGCCTTGTTCA
  GTCTTACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011880
• Insert Size: 1410 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011880.3, NP_036010.2
• RefSeq Size: 2424 bp
• RefSeq ORF: 1410 bp
• Locus ID: 24012
• UniProt ID: O54829
• Cytogenetics: 1 81.11 cM
• Gene Summary: Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. The RGS7/GNB5 dimer enhances GNAO1 GTPase activity. May play a role in synaptic vesicle exocytosis. Modulates the activity of potassium channels that are activated by GNAO1 in response to muscarinic acetylcholine receptor M2/CHRM2 signaling.[UniProtKB/Swiss-Prot Function]