Ficd (NM_001010825) Mouse Untagged Clone

CAT#: MC216196

Ficd (untagged) - Mouse FIC domain containing (Ficd), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ficd
• Synonyms: D5Ertd40e; Hype
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216196 representing NM_001010825
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATACTCATGCCAATGGCGTCGGTGGTGGCAGTGGCCGAACCCAAATGGGTCTCCGTCTGGGGCCGTT
  TCCTGTGGATGGCGCTGCTGAGCATGGCTCTGGGGTCGCTGCTGGCTCTGCTGCTGCCGCTGGGAGTTGT
  GGAAGAGCATTGCCTGGCTGTGCTCAGAGGTTTCCACCTGCTCAGGAGCAAACTGGACAGGGCACAGCCT
  GTGGTCCCCAAGTGCACCAGCCTATGCACAGAGCTCAGTGTCTCCTCCAGGGATGCAGGGCTGCTGACAG
  TCAAGACTACGGCGTCTCCAGCAGGGAAGCTGGAAGCCAAGGCCGCCCTAAACCAAGCCCTGGAGATGAA
  GCGTCAAGGCAAGAGAGGGAAAGCCCACAAGCTCTTCCTGCACGCCCTCAAGATGGACCCCGGCTTTGTA
  GACGCACTGAATGAGTTCGGCATCTTCTCCGAAGAAGACAAGGACATCATCCAGGCTGACTACTTATACA
  CCAGGGCCCTGACCATCTCGCCCTTCCACGAGAAAGCCCTGGTCAACCGGGATCGGACACTGCCCCTCGT
  GGAGGAAATCGACCAGAGGTACTTCAGCGTCATCGACAGCAAAGTGAAGAAGGTCATGTCCATCCCAAAG
  GGGAGCTCAGCGCTGCGCAGGGTCATGGAGGAGACCTACTACCACCACATCTACCACACGGTGGCCATCG
  AGGGCAACACCCTCACCCTCTCGGAGATCAGGCACATCCTGGAGACCCGCTACGCCGTGCCAGGGAAGAG
  CCTGGAAGAGCAGAACGAGGTGATCGGCATGCACGCGGCCATGAAGTACATCAACACCACCCTGGTCTCC
  CGCATCGGGTCTGTCACCATGGACGACATGCTGGAGATCCACAGGAGGGTACTGGGGTATGTGGATCCAG
  TGGAGGCGGGCAGGTTCCGGAGGACCCAGGTCCTGGTGGGCCACCACATCCCACCCCACCCCCGGGATGT
  GGAGAAGCAGATGCAGGAGTTCACACAGTGGCTCAATTCAGAGGACGCCATGAACCTGCACCCAGTCGAG
  TTCGCGGCCCTGGCCCATTACAAACTGGTGTACATCCACCCTTTCATCGACGGCAACGGGAGGACCTCCC
  GTCTGCTGATGAACCTGATTTTGATGCAGGCGGGATACCCGCCCATCACCATCCGCAAGGAGCAGAGGTC
  CGAGTACTACCATGTACTGGAAGTCGCCAACGAGGGTGACGTGCGGCCTTTCATCCGCTTCATAGCCAAG
  TGTACGGAGGTCACACTGGACACGTTGCTCCTTGCCACCACCGAGTACTCGGTGGCACTGCCAGAAGCCC
  AGCCCAACCATTCTGGGTTCAAGGAGACGCTCCCTGTGAGGCCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001010825
• Insert Size: 1377 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001010825.3, NP_001010825.2
• RefSeq Size: 3192 bp
• RefSeq ORF: 1377 bp
• Locus ID: 231630
• UniProt ID: Q8BIX9
• Cytogenetics: 5 55.55 cM
• Gene Summary: Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context (By similarity). The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place (By similarity). Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP (By similarity). In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it (By similarity). In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity (By similarity). Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates (By similarity).[UniProtKB/Swiss-Prot Function]