Tcf7l2 (NM_001142918) Mouse Untagged Clone

CAT#: MC216129

Tcf7l2 (untagged) - Mouse transcription factor 7-like 2, T-cell specific, HMG-box (Tcf7l2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tcf7l2
• Synonyms: Tcf-4; Tcf4; TCF4B; TCF4E
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216129 representing NM_001142918
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCAGCTGAACGGCGGTGGAGGAGATGACCTAGGCGCTAACGACGAGCTGATCTCCTTCAAAGACG
  AAGGCGAGCAGGAGGAGAAGAACTCGGAAAACTCCTCGGCGGAAAGGGATTTAGCCGATGTCAAGTCCTC
  GCTGGTCAATGAATCAGAGACGAATCAAAACAGCTCCTCCGATTCCGAGGCGGAAAGACGGCCTCCGCCT
  CGCTCCGAAAGTTTCCGAGATAAATCCCGGGAAAGTTTGGAAGAAGCGGCCAAGAGGCAAGATGGAGGGC
  TCTTTAAGGGGCCACCGTATCCCGGCTACCCCTTCATCATGATCCCCGACCTGACGAGCCCCTACCTCCC
  CAACGGATCGCTCTCGCCCACCGCCCGAACCTATCTTCAGATGAAATGGCCACTGCTTGATGTCCAAGCA
  GGAAGCCTCCAGAGCAGACAAACCCTCAAGGATGCTCGTTCGCCGTCGCCAGCACACATCGTTTCGAACA
  AAGTACCGGTGGTGCAACACCCCCACCATGTCCACCCACTCACGCCTCTCATCACGTACAGCAATGAACA
  CTTCACCCCGGGAAATCCACCTCCGCACTTACCAGCTGACGTAGACCCCAAAACAGGAATCCCAAGGCCT
  CCGCACCCTCCAGATATCTCTCCATATTACCCGCTGTCGCCCGGCACCGTAGGACAAATCCCCCATCCGC
  TAGGATGGTTAGTACCACAGCAAGGTCAGCCTGTGTACCCAATCACGACAGGAGGATTCAGACACCCCTA
  CCCCACAGCGCTGACAGTCAACGCATCTATGTCTAGGTTCCCTCCCCATATGGTCCCTCCCCATCACACT
  CTGCACACGACCGGCATCCCTCACCCGGCCATCGTCACACCGACAGTCAAGCAGGAATCCTCCCAGAGTG
  ACGTCGGCTCACTCCACAGCTCAAAGCATCAGGACTCCAAAAAGGAAGAAGAGAAGAAGAAGCCCCACAT
  AAAGAAGCCCCTTAATGCATTCATGTTGTATATGAAAGAGATGAGAGCGAAGGTGGTGGCCGAATGCACA
  TTGAAAGAGAGTGCAGCCATCAACCAGATTCTCGGGCGCAGGTGGCACGCCCTGTCCAGGGAAGAACAGG
  CAAAATACTACGAGCTGGCCCGGAAGGAACGACAGCTTCACATGCAGCTGTACCCTGGCTGGTCTGCACG
  GGATAACTATGGGAAGAAGAAGAAGAGAAAAAGAGACAAGCAGCCGGGGGAAACCAACGAACACAGCGAA
  TGTTTCCTAAATCCTTGCCTTTCGCTTCCTCCGATCACAGACCTGAGCGCTCCTAAGAAATGCCGAGCGC
  GCTTTGGCCTTGATCAACAGAATAACTGGTGCGGCCCCTGCAGTCTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001142918
• Insert Size: 1380 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142918.1, NP_001136390.1
• RefSeq Size: 4163 bp
• RefSeq ORF: 1380 bp
• Locus ID: 21416
• UniProt ID: Q924A0
• Cytogenetics: 19 51.59 cM
• Gene Summary: Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif CCTTTGATC in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1 (By similarity). Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.[UniProtKB/Swiss-Prot Function]