Gabra1 (NM_010250) Mouse Untagged Clone

CAT#: MC216110

Gabra1 (untagged) - Mouse gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1 (Gabra1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gabra1
• Synonyms: GABAA-alpha1; GABAAR-alpha1; Gabra-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216110 representing NM_010250
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAAAAGTCGGGGTCTCTCTGACTATCTTTGGGCCTGGACCCTCATTCTGAGCACACTGTCGGGAA
  GAAGCTATGGACAGCCCTCCCAAGATGAACTTAAAGACAACACCACTGTCTTCACAAGAATTTTGGACCG
  ACTGCTGGACGGTTATGACAATCGTCTGAGACCAGGTTTGGGAGAGCGTGTAACTGAAGTGAAGACCGAC
  ATTTTCGTCACCAGTTTCGGACCAGTTTCAGACCACGATATGGAGTATACAATAGATGTGTTTTTCCGTC
  AAAGTTGGAAGGATGAAAGATTAAAATTCAAAGGACCCATGACAGTGCTCCGGCTAAACAACCTTATGGC
  CAGTAAAATCTGGACTCCAGATACATTTTTCCACAATGGAAAGAAGTCTGTGGCCCACAACATGACCATG
  CCTAATAAGCTCCTGCGTATCACAGAGGATGGCACTCTGCTGTACACCATGAGGTTGACCGTGAGAGCTG
  AATGCCCAATGCACTTGGAAGACTTCCCTATGGATGCCCATGCCTGCCCACTAAAATTCGGAAGCTATGC
  TTATACAAGAGCAGAAGTTGTCTATGAGTGGACCAGAGAGCCAGCCCGTTCAGTGGTTGTAGCAGAAGAT
  GGGTCACGTTTAAACCAGTATGACCTTCTTGGACAAACAGTTGACTCTGGAATTGTTCAGTCCAGTACTG
  GAGAATATGTGGTTATGACGACTCACTTCCACTTGAAGAGAAAAATTGGCTACTTTGTTATTCAAACATA
  TCTGCCGTGCATAATGACAGTTATTCTCTCCCAAGTCTCCTTCTGGCTCAACAGAGAGTCAGTACCAGCA
  AGAACTGTCTTTGGAGTGACGACTGTTCTGACTATGACAACCTTGAGTATCAGTGCCAGAAATTCCCTCC
  CGAAGGTGGCTTATGCAACAGCTATGGACTGGTTTATTGCAGTATGCTATGCCTTTGTTTTCTCAGCTCT
  GATTGAGTTTGCCACAGTAAACTATTTCACCAAGAGAGGGTATGCGTGGGATGGCAAAAGCGTGGTTCCA
  GAAAAGCCAAAGAAAGTAAAGGATCCTCTCATTAAGAAAAACAACACATATGCTCCTACAGCAACCAGCT
  ATACCCCTAACTTAGCCAGGGGTGACCCCGGCTTGGCAACTATTGCTAAAAGTGCGACCATAGAACCGAA
  AGAAGTCAAGCCTGAGACAAAACCACCAGAACCCAAGAAAACCTTTAACAGCGTCAGCAAAATCGACCGA
  CTGTCAAGAATAGCCTTTCCGCTGCTATTTGGAATCTTTAACTTAGTCTATTGGGCCACGTATTTAAACA
  GAGAGCCTCAGCTAAAAGCCCCCACACCCCATCAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010250
• Insert Size: 1368 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010250.5, NP_034380.1
• RefSeq Size: 4702 bp
• RefSeq ORF: 1368 bp
• Locus ID: 14394
• UniProt ID: P62812
• Cytogenetics: 11 24.97 cM
• Gene Summary: Ligand-gated chloride channel which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:27129275). Plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel (PubMed:27129275). The gamma2 subunit is necessary but not sufficient for a rapid formation of active synaptic contacts and the synaptogenic effect of this subunit is influenced by the type of alpha and beta subunits present in the receptor pentamer (PubMed:27129275). The alpha1/beta2/gamma2 receptor and the alpha1/beta3/gamma2 receptor exhibit synaptogenic activity (PubMed:27129275). GABRA1-mediated plasticity in the orbitofrontal cortex regulates context-dependent action selection (PubMed:25348603). Functions also as histamine receptor and mediates cellular responses to histamine (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.