Gabra1 (NM_010250) Mouse Tagged ORF Clone

CAT#: MG227303

Gabra1 (tGFP-tagged) - Mouse gamma-aminobutyric acid (GABA) A receptor subunit alpha 1 (Gabra1), (10ug)

Product Images

Circular map for MG227303

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gabra1
• Synonyms: GABAA-alpha1; GABAAR-alpha1; Gabra-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227303 representing NM_010250
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAAAAGTCGGGGTCTCTCTGACTATCTTTGGGCCTGGACCCTCATTCTGAGCACACTGTCGGGAA
  GAAGCTATGGACAGCCCTCCCAAGATGAACTTAAAGACAACACCACTGTCTTCACAAGAATTTTGGACCG
  ACTGCTGGACGGTTATGACAATCGTCTGAGACCAGGTTTGGGAGAGCGTGTAACTGAAGTGAAGACCGAC
  ATTTTCGTCACCAGTTTCGGACCAGTTTCAGACCACGATATGGAGTATACAATAGATGTGTTTTTCCGTC
  AAAGTTGGAAGGATGAAAGATTAAAATTCAAAGGACCCATGACAGTGCTCCGGCTAAACAACCTTATGGC
  CAGTAAAATCTGGACTCCAGATACATTTTTCCACAATGGAAAGAAGTCTGTGGCCCACAACATGACCATG
  CCTAATAAGCTCCTGCGTATCACAGAGGATGGCACTCTGCTGTACACCATGAGGTTGACCGTGAGAGCTG
  AATGCCCAATGCACTTGGAAGACTTCCCTATGGATGCCCATGCCTGCCCACTAAAATTCGGAAGCTATGC
  TTATACAAGAGCAGAAGTTGTCTATGAGTGGACCAGAGAGCCAGCCCGTTCAGTGGTTGTAGCAGAAGAT
  GGGTCACGTTTAAACCAGTATGACCTTCTTGGACAAACAGTTGACTCTGGAATTGTTCAGTCCAGTACTG
  GAGAATATGTGGTTATGACGACTCACTTCCACTTGAAGAGAAAAATTGGCTACTTTGTTATTCAAACATA
  TCTGCCGTGCATAATGACAGTTATTCTCTCCCAAGTCTCCTTCTGGCTCAACAGAGAGTCAGTACCAGCA
  AGAACTGTCTTTGGAGTGACGACTGTTCTGACTATGACAACCTTGAGTATCAGTGCCAGAAATTCCCTCC
  CGAAGGTGGCTTATGCAACAGCTATGGACTGGTTTATTGCAGTATGCTATGCCTTTGTTTTCTCAGCTCT
  GATTGAGTTTGCCACAGTAAACTATTTCACCAAGAGAGGGTATGCGTGGGATGGCAAAAGCGTGGTTCCA
  GAAAAGCCAAAGAAAGTAAAGGATCCTCTCATTAAGAAAAACAACACATATGCTCCTACAGCAACCAGCT
  ATACCCCTAACTTAGCCAGGGGTGACCCCGGCTTGGCAACTATTGCTAAAAGTGCGACCATAGAACCGAA
  AGAAGTCAAGCCTGAGACAAAACCACCAGAACCCAAGAAAACCTTTAACAGCGTCAGCAAAATCGACCGA
  CTGTCAAGAATAGCCTTTCCGCTGCTATTTGGAATCTTTAACTTAGTCTATTGGGCCACGTATTTAAACA
  GAGAGCCTCAGCTAAAAGCCCCCACACCCCATCAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227303 representing NM_010250
  Red=Cloning site Green=Tags(s)
  
  MKKSRGLSDYLWAWTLILSTLSGRSYGQPSQDELKDNTTVFTRILDRLLDGYDNRLRPGLGERVTEVKTD
  IFVTSFGPVSDHDMEYTIDVFFRQSWKDERLKFKGPMTVLRLNNLMASKIWTPDTFFHNGKKSVAHNMTM
  PNKLLRITEDGTLLYTMRLTVRAECPMHLEDFPMDAHACPLKFGSYAYTRAEVVYEWTREPARSVVVAED
  GSRLNQYDLLGQTVDSGIVQSSTGEYVVMTTHFHLKRKIGYFVIQTYLPCIMTVILSQVSFWLNRESVPA
  RTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKRGYAWDGKSVVP
  EKPKKVKDPLIKKNNTYAPTATSYTPNLARGDPGLATIAKSATIEPKEVKPETKPPEPKKTFNSVSKIDR
  LSRIAFPLLFGIFNLVYWATYLNREPQLKAPTPHQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010250
• ORF Size: 1365 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010250.5, NP_034380.1
• RefSeq ORF: 1368 bp
• Locus ID: 14394
• UniProt ID: P62812
• Cytogenetics: 11 24.97 cM
• Gene Summary: Ligand-gated chloride channel which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:27129275). Plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel (PubMed:27129275). The gamma2 subunit is necessary but not sufficient for a rapid formation of active synaptic contacts and the synaptogenic effect of this subunit is influenced by the type of alpha and beta subunits present in the receptor pentamer (PubMed:27129275). The alpha1/beta2/gamma2 receptor and the alpha1/beta3/gamma2 receptor exhibit synaptogenic activity (PubMed:27129275). GABRA1-mediated plasticity in the orbitofrontal cortex regulates context-dependent action selection (PubMed:25348603). Functions also as histamine receptor and mediates cellular responses to histamine (By similarity).[UniProtKB/Swiss-Prot Function]