Gabra6 (NM_001099641) Mouse Untagged Clone

CAT#: MC215936

Gabra6 (untagged) - Mouse gamma-aminobutyric acid (GABA) A receptor, subunit alpha 6 (Gabra6), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gabra6
• Synonyms: alpha6; GABA-ARalpha6; Gabra-6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215936 representing NM_001099641
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCTTGCTTCTCCCCTGGCTCTTCATTATTCTATGGCTAGAAAATGCCCAAGCTCAACTTGAAGATG
  AAGGCAACTTCTACTCTGAAAATGTCAGTCGGATTCTTGACAACTTGCTGGAAGGCTATGACAACCGTCT
  ACGGCCAGGATTTGGGGGTGCTGTAACAGAAGTCAAAACAGACATCTATGTGACCAGCTTTGGACCTGTG
  TCAGATGTGGAGATGGAGTATACAATGGATGTTTTCTTCCGCCAGACTTGGACTGATGAGAGACTGAAAT
  TTAAAGGCCCTGCTGAGATTCTGAGCTTAAATAACTTGATGGTCAGTAAGATCTGGACTCCTGACACTTT
  TTTCCGGAATGGGAAAAAGTCAATTGCTCACAACATGACCACCCCCAACAAGCTCTTCCGATTAATGCAG
  AATGGAACAATCCTCTATACCATGAGGCTCACCATCAACGCTGATTGCCCCATGAGATTGGTTAACTTCC
  CTATGGATGGACACGCTTGTCCACTCAAGTTTGGGAGCTATGCTTATCCTAAAACTGAAATCATATATAC
  ATGGAAAAAAGGACCACTTTACTCAGTAGAAGTCCCAGAAGAATCTTCTAGCCTCCTCCAGTATGATTTG
  ATTGGGCAAACAGTTTCTAGTGAGACAATTAAATCTAACACTGGTGAATATGTAATCATGACAGTCTATT
  TCCACTTACAAAGGAAGATGGGCTATTTCATGATCCAGATATACACTCCGTGCATCATGACAGTCATTCT
  TTCTCAAGTGTCTTTCTGGATTAATAAGGAGTCAGTCCCAGCAAGAACTGTCTTTGGAATCACCACTGTT
  TTAACCATGACCACCTTAAGCATCAGTGCTCGGCACTCTCTACCCAAAGTGTCCTATGCAACCGCCATGG
  ATTGGTTCATAGCTGTATGCTTTGCTTTTGTCTTTTCTGCTCTCATTGAATTCGCAGCTGTCAACTACTT
  CACCAATCTCCAGTCACAGAAGGCTGAAAGGCAGGCACAAACTGCAGCCACGCCCCCTGTGGCAAAGTCA
  AAAGCGTCTGAATCCCTGCAAGCAGAGATTGTTGTGCATTCTGACTCCAAGTACCATCTGAAGAAGAGAA
  TCAGCTCTCTGACTTTGCCCATCGTTCCATCTTCTGAGGCCAGCAAAGCCCTCAGTAGAACGCCCATCTT
  AAAATCGACGCCTGTCTCTCCCCCATTGCTCTTACCAGCCACTGGGGGCACCAGCAAAATAGATCAGTAT
  TCTCGAATTCTCTTCCCAGTAGCTTTTGCAGGATTCAACCTTGTGTACTGGATAGTTTACCTTTCCAAAG
  ATACAATGGAAGTGAGCAGTACTGTTGAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001099641
• Insert Size: 1362 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001099641.2, NP_001093111.1
• RefSeq Size: 2505 bp
• RefSeq ORF: 1362 bp
• Locus ID: 14399
• UniProt ID: P16305
• Cytogenetics: 11 25.03 cM
• Gene Summary: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).