Gfral (NM_205844) Mouse Untagged Clone

CAT#: MC214864

Gfral (untagged) - Mouse GDNF family receptor alpha like (Gfral), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gfral
• Synonyms: AY457637; Gral
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC214864 representing NM_205844
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTAGTGTTCATTTTCCTGGCTGTTACGTTAAGCTCAGAAAATGAATCCTCTTCCCAAACAAATGATT
  GTGCACATTTAATACAGAAATGCTTGATTGATGCAAATGGCTGTGAGCAGTCATGGAGATCAATGGAAGA
  CACCTGCCTTACTCCAGGTGACTCCTGCAAGATAAATAATTCACTACATTGTAACCTGAGTATCCAGGCT
  TTGGTGGAAAAAAATTTCCAATTTAAAGAGTGTCTTTGTATGGATGACCTCCACTGTACAGTAAACAAAC
  TTTTTGGAAAAAAGTGCACCAATAAGACAGATAACATGGAAAAGGACAATAAAGATAAATGGAATCTAAC
  TACTACTCCTTTCTATCATGGATTCAAACAGATGCAGTCTTGTTTGGAGGTGACAGAGGCGTGTGTAGGG
  GATGTGGTTTGTAATGCACAGTTGGCCCTTTACCTTAAAGCATGCTCAGCAAATGGAAATCTGTGTGATG
  TGAAACACTGCCAAGCAGCCATACGGTTCTTCTATCAAAATATGCCTTTTAACACTGCCCAGATGTTGGC
  TTTTTGTGACTGTGCTCAATCTGATATACCCTGTCAGCAATCCAAAGAAACTCTTCACAGCAAGCCATGT
  GCACTGAATATAGTTCCACCCCCCACTTGCCTCAGTGTAATTCACACTTGCCGAAATGATGAATTATGCA
  GGACACACTACCGAACATTCCAGACAGAATGCTGGCCCCACATAACTGGGAAGTGCCATGAAGATGAGAC
  CTGCATTAGCATGTTAGGCAAGCAAGACCTTACTTGTTCTGGGAGTGAGAGCTGCAGGGCTGCCTTCCTA
  GGAACCTTTGGGACAGTCCTGCAAGTACCCTGTGCTTGCAGGGGCGTTACACAGGCTGAAGAACACGTGT
  GCATGATTTTCCAGCACATGCTTCATAGCAAATCGTGTTTCAATTACCCAACTCCTAATGTCAAAGACAT
  TTCCTCATATGAAAAAAAGAATTCAAAAGAAATTACTCTGACTGGATTCAATTCTTTCTTCAATGGAGAA
  CTACTCTATGTTGTTGTGTGCATGGCAGTTACCTGTGGAATTCTTTTCTTGGTGATGCTCAAGTTAAGGA
  TACAAAGTGAAAAAAGAGATCCCTCATCCATCGAAATAGCTGGAGGTGTCATCATTCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_205844
• Insert Size: 1182 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_205844.3, NP_995316.2
• RefSeq Size: 2080 bp
• RefSeq ORF: 1182 bp
• Locus ID: 404194
• UniProt ID: Q6SJE0
• Cytogenetics: 9 D
• Gene Summary: Brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses (PubMed:28953886, PubMed:28846099, PubMed:28846097, PubMed:28846098). Upon interaction with its ligand, GDF15, interacts with RET and induces cellular signaling through activation of MAPK- and AKT- signaling pathways (PubMed:28846098, PubMed:28846099).[UniProtKB/Swiss-Prot Function]