Opn5 (NM_181753) Mouse Untagged Clone

CAT#: MC214652

Opn5 (untagged) - Mouse opsin 5 (Opn5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Opn5
• Synonyms: Gpr136; Neuropsin; PGR12; TMEM13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC214652 representing NM_181753
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTGAACCACACTGCCCTACCTCAGGATGAGCGCCTGCCCCACTATCTTCGAGACGAGGACCCTT
  TTGCTTCCAAACTTTCCTGGGAAGCGGATTTAGTGGCTGGCTTTTACCTAACAATAATCGGGATTCTCTC
  TACATTTGGAAATGGGTATGTCCTTTATATGTCTTCTAGACGCAAGAAGAAGCTGAGACCTGCGGAAATA
  ATGACTATCAATTTAGCAGTCTGTGATCTGGGGATATCAGTTGTAGGCAAGCCGTTCACCATCATCTCTT
  GCTTCTGCCACCGCTGGGTGTTTGGCTGGTTTGGCTGCCGCTGGTATGGCTGGGCTGGATTTTTCTTTGG
  CTGTGGAAGCCTGATTACCATGACTGCTGTCAGCCTGGACCGCTATCTGAAGATCTGTTATCTGTCTTAT
  GGGGTCTGGCTGAAGAGAAAGCATGCCTACATCTGCCTGGCAGTCATCTGGGCTTATGCTTCCTTCTGGA
  CCACCATGCCCTTGGTGGGCCTGGGGGACTATGCACCTGAGCCCTTCGGAACCTCATGCACCCTGGACTG
  GTGGCTGGCCCAGGCTTCAGGTGGGGGTCAGGTGTTCATCCTGAGCATCCTCTTCTTCTGCCTCCTGCTG
  CCAACGGCTGTGATTGTTTTCTCATATGCTAAGATCATCGCCAAGGTGAAGTCTTCTTCTAAAGAGGTAG
  CCCATTTCGACAGTCGAATCCATAGCAGCCATGTACTTGAGGTGAAGCTGACCAAGGTGGCAATGCTGAT
  TTGTGCTGGTTTCCTGATTGCCTGGATTCCTTATGCGGTCGTCTCTGTGTGGTCAGCTTTTGGAAGGCCA
  GACTCCATCCCCATACAGCTCTCCGTGGTGCCCACACTCCTTGCAAAATCAGCAGCGATGTACAATCCAA
  TCATCTACCAGGTCATTGATTACAGATTTGCCTGTTGCCAGGCTGGTGGTTTGAGAGGAACGAAGAAGAA
  ATCTTTGGAAGACTTTAGGCTGCATACTGTAACCGCAGTCAGGAAGTCTTCTGCTGTGCTGGAGATCCAT
  CCAGAGAGCAGTTCCAGATTTACTAGTGCCCATGTTATGGATGGAGAGAGTCACAGTAATGATGGTGACT
  GTGGCAAGAAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_181753
• Insert Size: 1134 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181753.4, NP_861418.2
• RefSeq Size: 1824 bp
• RefSeq ORF: 1134 bp
• Locus ID: 353344
• UniProt ID: Q6VZZ7
• Cytogenetics: 17 B3
• Gene Summary: G-protein coupled receptor which selectively activates G(i) type G proteins via ultraviolet A (UVA) light-mediated activation in the retina (PubMed:22043319). Preferentially binds the chromophore 11-cis retinal and is a bistable protein that displays emission peaks at 380 nm (UVA light) and 470 nm (blue light) (PubMed:22043319, PubMed:31607531). Required for the light-response in the inner plexiform layer, and contributes to the regulation of the light-response in the nerve fiber layer, via phosphorylated DAT/SLC6A3 dopamine uptake (PubMed:30936473). Involved in local corneal and retinal circadian rhythm photoentrainment via modulation of the UVA light-induced phase-shift of the retina clock (PubMed:26392540, PubMed:30240620). Acts as a circadian photoreceptor in the outer ear and vibrissal pads, via modulation of circadian clock-gene expression in response to violet light during the light-to-dark transition phase and night phase of the circadian cycle (PubMed:31607531). Required in the retina to negatively regulate hyaloid vessel regression during postnatal development via light-dependent OPN5-SLC32A1-DRD2-VEGFR2 signaling (PubMed:30936473). Involved in the light-dependent regulation of retina and vitreous compartment dopamine levels (PubMed:30936473).[UniProtKB/Swiss-Prot Function]