Shisa3 (NM_001033415) Mouse Untagged Clone

CAT#: MC214574

Shisa3 (untagged) - Mouse shisa homolog 3 (Xenopus laevis) (Shisa3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Shisa3
• Synonyms: D830007B15Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC214574 representing NM_001033415
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGCGCTGCTGGCGTTCTGTCTGCTCGTGGGCTTGCTACGTTGGGGCCCGGCGGGCGCGCAGCAGC
  CTGGGGAATACTGCCACGGCTGGGTGGACGCGCAGGGCAACTATCACGAGGGGTTCCAGTGCCCCGAGGA
  CTTCGACACGCAGGACGCCACCATCTGCTGCGGCTCGTGTGCGCTGCGCTACTGCTGCGCGGCAGCCGAC
  GCCAGGTTGGAGCAGGGGGGCTGCACCAACGACCGCGGAGAGCTGGAGCATCCTGGCATCACCGCGCAAC
  CTGTCTATGTCCCCTTCCTGATCGTCGGCTCCATCTTCATCGCCTTCATCATTTTGGGCTCTTTAGTGGC
  TATCTATTGTTGCACCTGTTTGAGACCCAAGGAGCCCTCTCAGCAGCCCATCCGCTTCTCGCTCCGAAGC
  TATCAGACAGAGACCTTGCCCATGATCCTCACCTCCACCAGCCTCAGAGCAGCTTCCAGGCAGTCCAGCA
  CCGCTACCAGCTCCAGCTCCACGGGAGGATCTGTGCGGAGGTTCTCCTTTGCCAGGGCTGAGCCAAGCTG
  CCTGGTGCCCTCATCACCCCCACCTTACACCACTGGTCACACCATACACCTAACCCAACCGTCCGGTTTC
  CTGGTATCACCCCAGTATTTTGCATACCCTCTCCAGCAGGAGCCCCCATTACCTGGGAAGAGCTGTCCAG
  ATTTCAGTTCCAGTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033415
• Insert Size: 717 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033415.3, NP_001028587.1
• RefSeq Size: 3754 bp
• RefSeq ORF: 717 bp
• Locus ID: 330096
• UniProt ID: Q3UPR0
• Cytogenetics: 5 C3.1
• Gene Summary: Plays an essential role in the maturation of presomitic mesoderm cells by individual attenuation of both FGF and WNT signaling.[UniProtKB/Swiss-Prot Function]