Gpr68 (NM_001177674) Mouse Untagged Clone

CAT#: MC213054

Gpr68 (untagged) - Mouse G protein-coupled receptor 68 (Gpr68), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gpr68
• Synonyms: BB131428; Ogr1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC213054 representing NM_001177674
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAACATCACTACAGAAAACTCCTCACTATCTTGCCCCATCGACCACACCATCCACCAGACACTAG
  CCCCAGTGGTCTATGTGACCGTGCTGGTGGTGGGCTTCCCAGCCAACTGCCTGTCCCTCTACTTCGGGTA
  CTTGCAGATCAAGGCCCGGAATGAGCTGGGAGTGTACCTGTGTAACCTGACCATTGCAGACCTGTTCTAT
  ATCTGTTCACTTCCCTTCTGGCTGCAGTACGTGCTTCAGCACGACGACTGGTCCCATGGTGACCTATCCT
  GCCAGGTGTGTGGCATCCTCCTCTATGAGAACATTTACATCAGCGTGGGCTTCCTCTGCTGCATCTCCAT
  CGACCGCTACCTGGCTGTGGCCCACCCCTTCCGCTTCCACCAGTTCCGCACCCTGAAGGCAGCCGTGGGT
  GTCAGTGTGCTCATCTGGGCCAAGGAGCTGCTGACCAGCATCTACTTCCTCAATCACAAGGAGGTCATTG
  AGGACGAGGACCAGCACCGAGTCTGCTTTGAGCATTACCCTATCCAGGCCTGGCAGCGTAGCATCAACTA
  CTACCGCTTCCTGGTGGGCTTTCTCTTCCCCATCTGCCTGCTGCTGGCCTCCTACCAGGGCATCCTGCGG
  GCTGTGCGCCGCAGCCACGGCACACAGAAGAGCCGCAAGGACCAGATTCAGCGGCTGGTGCTCAGCACCG
  TGGTCATCTTCCTGGCTTGCTTTCTACCCTACCACGTGCTGCTGCTGGTACGCAGCCTCTGGGAGAGAAA
  CTGTGAGTTTGCCAAGAGCATCTTCAACGTCTATCACTTCTCCCTCCTCCTCACCAGCTTCAACTGTGTA
  GCTGACCCGGTGCTGTACTGCTTTGTCAGTGAGACCACTCACAGGGACCTAGCCCGCCTCCGAGGAGCCT
  GCCTAGCTGTCCTTACCTGCTCTAGGACAAGCAGGGCCAGGGAGGCCTACCCTCTGGGTGCCCCTGAGGC
  CTCTGGGAAAAGTGGGGCCCAGGGCGAGGAACCTGAATTGTTAACCAAGCTCCACTCAGCCTTCCAGACC
  CCTAGCTCACTGGGAGTGGGAGGGCCCTCCACAGTGGGGTTGGCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001177674
• Insert Size: 1098 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177674.1, NP_001171145.1
• RefSeq Size: 3120 bp
• RefSeq ORF: 1098 bp
• Locus ID: 238377
• UniProt ID: Q8BFQ3
• Cytogenetics: 12 E
• Gene Summary: Proton-sensing receptor involved in pH homeostasis. May represents an osteoblastic pH sensor regulating cell-mediated responses to acidosis in bone. Mediates its action by association with G proteins that stimulates inositol phosphate (IP) production or Ca(2+) mobilization. The receptor is almost silent at pH 7.8 but fully activated at pH 6.8. Function also as a metastasis suppressor gene in prostate cancer.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.