Spry3 (NM_001030293) Mouse Untagged Clone

CAT#: MC213021

Spry3 (untagged) - Mouse sprouty homolog 3 (Drosophila) (Spry3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Spry3
• Synonyms: Gm391; Gm1409; sprouty3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC213021 representing NM_001030293
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCCACAGTGATAGATGAGCTCCAACAAATTCTGCCTATTGAACAGCTGCGCTCTACTCACGCTA
  GCAATGATTATGTGGAACGGCCTCCAGCACCCTGTAAACAAGCTCTTTCTAGCCCTTCCCTCATTGTGCA
  AACCCACAAATCTGATTGGTCCCTGGCTACCATGCCTACTGCTCTCCCACGCAGTATCAGCCAGTGCCAT
  CAGCTGCAGCCCCTGCCTCAGCATCTGAGCCAGTCTAGTATTTCCAGCTCAATGTCCCAAAGCACCACTG
  CCTCTGATCAAAGGCTCTTGGCCAGCATTACACCCTCTCCTTCAGGCCAGTCCATCATCAGAACCCAGCC
  TGGAGCAGGGGCCCACCCAAAGGTCGATGGTGCTCTGAAGGGAGAAGCTGAGCAATCTGTAGGGCATTCC
  AGTGATCACCTCTTTATCTGCGAGGAGTGTGGGCGCTGCAAGTGTGTCCCTTGCACAGCGGTTCGCCCTC
  TGCCCTCCTGCTGGATGTGCAACCAGCGTTGCCTTTGCTCTGCTGAGAGCCTCCTTGATTATGGCACTTG
  CCTCTGCTGTGTCAAGGGCCTCTTCTACCACTGTTCCACTGATGATGAAGACAACTGCGCTGATGAGCCC
  TGCTCCTGTGGGCCTAGCTCTTGCTTCATTCGCTGGGCAGCCATGAGTCTCATCTCCCTTTTCTTACCCT
  GCCTGTGCTGCTACCTGCCTACACGTGGATGCCTCCATATGTGCCAGCAGGGCTATGATAGCCTCCGTCG
  ACCAGGCTGCCGCTGTAAGAGGCACACCAACACTGTGTGCAGAAAAATCTCTTCTAGCAGTTCACCCTTC
  CCCAAGGCGCAAGAAAAGTCTGTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001030293
• Insert Size: 867 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001030293.2, NP_001025464.1
• RefSeq Size: 4542 bp
• RefSeq ORF: 867 bp
• Locus ID: 236576
• UniProt ID: Q3UUD2
• Cytogenetics: X
• Gene Summary: Inhibits neurite branching, arbor length and neurite complexity (PubMed:26089202). Inhibits EGF-mediated p42/44 ERK signaling (PubMed:16877379). Negatively regulates the MAPK cascade, resulting in a reduction of extracellular matrix protein accumulation (By similarity). May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis (By similarity).[UniProtKB/Swiss-Prot Function]