Yeats2 (NM_001145931) Mouse Untagged Clone

CAT#: MC212496

Yeats2 (untagged) - Mouse YEATS domain containing 2 (Yeats2), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Yeats2
• Synonyms: BC042768; mKIAA1197
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212496 representing NM_001145931
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGGAATCAAGCGAACAATCAAAGAAACTGACCCTGATTATGAGGATGTATCTGTGGCCCTTCCAA
  ATAAGCGGCATAAAGCGATTGAGAGTTCAGCTCGAGATGCAGCTGTGCAGAAAATTGAGACTATTATTAA
  GGAGCAGTTTGCTCTTGAGATGAAGAATAAGGAACATGAGATTGATGTCATTGACCAGCGACTGATTGAA
  GCCAGGAGGATGATGGATAAGCTCCGGGCCTGCATTGTAGCAAACTATTATGCCTCCGCTGGTCTCCTGA
  AGGTTTCTGAGGGATTAAAGACATTTGATCCGATGGCTTTTAATCACCCTGCTATCAAGAAATTTTTGGA
  ATCACCTTCTAGGTCATCATCTCCTACCAATCAGAGATCAGAAACACCATCTGCCAATCACTCTGAAAGT
  GACTCTTTATCTCAGCACAATGACTTCCTGTCTGACAAAGACAATAACAGCAACGTGGATGTGGAGGAAA
  GACCCCCAAGCACCGGGGAACAGCGACCGAGCCGAAAGGCAGGAAGGGACACGTCTAGTATCAGTGGCTC
  CCACAAACGGGAACTACGGAATGCTGACCTCACAGGAGATGAGACTTCACGACTGTTTGTCAAGAAAACG
  ATAGTAGTCGGCAACGTGTCCAAGTACATACCTCCAGATAAGCGGGAAGAAAATGACCAGTCAACCCATA
  AGTGGATGGTATATGTCCGAGGGTCGCGTAGAGAGCCCAGCATTAATCACTTTGTCAAGAAAGTTTGGTT
  TTTCCTTCATCCTAGCTATAAACCAAACGATCTTGTGGAAGTTAGAGAGCCCCCCTTTCATCTGACTAGA
  AGAGGCTGGGGTGAGTTTCCTGTCAGAGTGCAAGTTCACTTTAAGGACAGCCAGAACAAGCGGATAGATA
  TCATACACAACCTGAAGGTACTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001145931
• Insert Size: 936 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145931.1, NP_001139403.1
• RefSeq Size: 2347 bp
• RefSeq ORF: 936 bp
• Locus ID: 208146
• Cytogenetics: 16 A3
• Gene Summary: Chromatin reader component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. YEATS2 specifically recognizes and binds histone H3 crotonylated at 'Lys-27' (H3K27cr). Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) includes an alternate segment at the 3' end compared to variant 1. This results in a frame-shift and a shorter isoform (3) with a distinct C-terminus compared to isoform 1.