Mchr1 (NM_145132) Mouse Untagged Clone

CAT#: MC212490

Mchr1 (untagged) - Mouse melanin-concentrating hormone receptor 1 (Mchr1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mchr1
• Synonyms: AW049955; Gpr24; Mch1r
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212490 representing NM_145132
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCTGCAAGCCTCGTTGCTGTCCACTGGCCCCAATGCCAGCAACATCTCCGATGGCCAGGATAATT
  TCACATTGGCGGGGCCACCTCCTCGCACAAGGAGTGTCTCCTACATCAACATCATCATGCCTTCAGTGTT
  TGGTACCATCTGTCTCCTGGGCATTGTGGGAAACTCCACAGTCATTTTTGCCGTGGTGAAGAAATCCAAG
  CTGCACTGGTGCAGCAACGTCCCTGACATCTTCATCATCAACCTCTCTGTGGTGGATCTGCTTTTCCTGC
  TGGGCATGCCTTTCATGATCCACCAGCTCATGGGTAATGGTGTCTGGCACTTTGGGGAAACCATGTGCAC
  CCTCATCACAGCCATGGACGCCAACAGTCAGTTCACCAGCACCTACATCCTGACTGCTATGGCCATTGAC
  CGCTACTTGGCCACCGTCCATCCCATCTCCTCCACCAAGTTCCGGAAGCCCTCCATGGCCACCCTGGTGA
  TCTGCCTCCTGTGGGCTCTCTCGTTCATTAGCATCACTCCTGTGTGGCTCTATGCCAGGCTTATCCCCTT
  CCCAGGGGGTGCTGTGGGCTGTGGCATCCGCCTACCAAACCCAGATACTGATCTTTACTGGTTCACTCTG
  TATCAGTTTTTCCTGGCCTTCGCCCTTCCGTTTGTGGTCATCACTGCTGCGTACGTGAAAATACTACAGC
  GCATGACGTCTTCGGTGGCCCCAGCCTCTCAACGCAGCATCCGGCTTCGGACAAAGAGGGTGACCCGCAC
  AGCCATTGCCATCTGTCTGGTCTTCTTTGTGTGCTGGGCGCCCTACTACGTGCTGCAGCTGACCCAGTTG
  TCCATCAGCCGCCCGACCCTCACATTCGTCTACCTGTACAATGCGGCTATCAGCTTGGGCTATGCCAACA
  GCTGCCTCAATCCCTTTGTGTACATAGTACTCTGTGAGACCTTTCGAAAACGCTTGGTGCTGTCGGTGAA
  GCCCGCGGCCCAGGGGCAGCTTCGCACGGTCAGCAATGCTCAGACAGCTGACGAGGAGAGGACAGAAAGC
  AAAGGCACCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145132
• Insert Size: 1062 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145132.2, NP_660114.1
• RefSeq Size: 2205 bp
• RefSeq ORF: 1062 bp
• Locus ID: 207911
• UniProt ID: Q8JZL2
• Cytogenetics: 15 E1
• Gene Summary: Receptor for melanin-concentrating hormone, coupled to both G proteins that inhibit adenylyl cyclase and G proteins that activate phosphoinositide hydrolysis.[UniProtKB/Swiss-Prot Function]