Skint3 (NM_177578) Mouse Untagged Clone

CAT#: MC212476

Skint3 (untagged) - Mouse selection and upkeep of intraepithelial T cells 3 (Skint3), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Skint3
• Synonyms: A430090E18Rik; Skint-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212476 representing NM_177578
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGATCTATACAAATAATTTTTGCTGCATACTGTGTTGTCTTGTGCGTCCTCCAGATGTTAGTACTGA
  GTTCAGAGCAGTTCACAATTACTGGCTTAGAGAGGCCAGTCCTTGCCCCATTAGGTGGAATCCTTGAACT
  CAGTTGTCAGTTGTCCCCACCACAAAATGCACAACAAATGGAGATTCGCTGGTTCCGGAATCGCTATACA
  GAACCTGTGTACCTGTACAGGAATGGTAAAGACCTGCATGGAGAAACTATCTCCAAGTATGTGGAGCGGA
  CAGAACTCCTAAAACATGACATTGGAAAAGGGAAAGTGACCCTCAGGGTCTTTAAAGTGACAGTTGATGA
  TGATGGATCATACCATTGTGTCTTCAAAGATGGTATATTCTATGAAGAGCATATCACAGAGGTCAAGGTC
  ACTGCCACAAGCTCAGATATAAAGATTATAATGCATCCCCCGAATATTAAAGGTGTGATGCTTGAGTGTC
  ATTCAAGAGGTTGGTTCCCTCAGCCTCATATGGAATGGAGAGATAGCAATGGACAGGTCATTCCAGCTAC
  ATCAAAATCCCAGTCACAGGATGAAAACAAATTGTTCAACATGACAATGAACCTTTTTGCTGATGTTGGC
  CTACACCAGATTGTCACTTGCTACATTCAAAACCTTTTAACTCATCAAGAAGAAAGCATAAGTATTGTCC
  TAACAGTCCCAATTTCAGATCAACATTTTGAATTGGATACTTTATACTTGGAAGATATTAGTGTGATCCT
  GTGTGTGGTGATTGTGTTTAACCTCAAGCTCAATTTACTTACTTACTACAGATTGGAACGTAAATATGAT
  GGATGTACACCTGGATGCAAAGCATGCTTCTATATATTAAAAATAATAATAATAATATTGCCATTCGTGT
  TTACCTTTGGGTGCTATAATGCAATCTTTTTAAAATATCATCAATTACAGAAAAAAGTTTCTATTCCAGA
  TCCCTTATATTACTTCTACACTAGCTGGCTTGTGAATATGGAAATGCTGGGAGTATTTCTGGTCTTCTTT
  CCTACTTTTATAAACTTAATAGAATTTTCGCAATTCATAAAAACTGTTCCAAAGCCCATATGGTTATGCC
  AAGAGAATATGAGAGAAGATGATGCAATAAGACACAGATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177578
• Insert Size: 1161 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177578.4, NP_808246.1
• RefSeq Size: 3627 bp
• RefSeq ORF: 1161 bp
• Locus ID: 195564
• UniProt ID: A7TZF0
• Cytogenetics: 4 D1
• Gene Summary: May act by engaging a cell surface molecule on immature T-cells in the embryonic thymus.[UniProtKB/Swiss-Prot Function]