Skint3 (NM_177578) Mouse Tagged ORF Clone

CAT#: MG224421

Skint3 (tGFP-tagged) - Mouse selection and upkeep of intraepithelial T cells 3 (Skint3) transcript variant 2, (10ug)

Product Images

Circular map for MG224421

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Skint3
• Synonyms: A430090E18Rik; Skint-3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224421 representing NM_177578
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGATCTATACAAATAATTTTTGCTGCATACTGTGTTGTCTTGTGCGTCCTCCAGATGTTAGTACTGA
  GTTCAGAGCAGTTCACAATTACTGGCTTAGAGAGGCCAGTCCTTGCCCCATTAGGTGGAATCCTTGAACT
  CAGTTGTCAGTTGTCCCCACCACAAAATGCACAACAAATGGAGATTCGCTGGTTCCGGAATCGCTATACA
  GAACCTGTGTACCTGTACAGGAATGGTAAAGACCTGCATGGAGAAACTATCTCCAAGTATGTGGAGCGGA
  CAGAACTCCTAAAACATGACATTGGAAAAGGGAAAGTGACCCTCAGGGTCTTTAAAGTGACAGTTGATGA
  TGATGGATCATACCATTGTGTCTTCAAAGATGGTATATTCTATGAAGAGCATATCACAGAGGTCAAGGTC
  ACTGCCACAAGCTCAGATATAAAGATTATAATGCATCCCCCGAATATTAAAGGTGTGATGCTTGAGTGTC
  ATTCAAGAGGTTGGTTCCCTCAGCCTCATATGGAATGGAGAGATAGCAATGGACAGGTCATTCCAGCTAC
  ATCAAAATCCCAGTCACAGGATGAAAACAAATTGTTCAACATGACAATGAACCTTTTTGCTGATGTTGGC
  CTACACCAGATTGTCACTTGCTACATTCAAAACCTTTTAACTCATCAAGAAGAAAGCATAAGTATTGTCC
  TAACAGTCCCAATTTCAGATCAACATTTTGAATTGGATACTTTATACTTGGAAGATATTAGTGTGATCCT
  GTGTGTGGTGATTGTGTTTAACCTCAAGCTCAATTTACTTACTTACTACAGATTGGAACGTAAATATGAT
  GGATGTACACCTGGATGCAAAGCATGCTTCTATATATTAAAAATAATAATAATAATATTGCCATTCGTGT
  TTACCTTTGGGTGCTATAATGCAATCTTTTTAAAATATCATCAATTACAGAAAAAAGTTTCTATTCCAGA
  TCCCTTATATTACTTCTACACTAGCTGGCTTGTGAATATGGAAATGCTGGGAGTATTTCTGGTCTTCTTT
  CCTACTTTTATAAACTTAATAGAATTTTCGCAATTCATAAAAACTGTTCCAAAGCCCATATGGTTATGCC
  AAGAGAATATGAGAGAAGATGATGCAATAAGACACAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224421 representing NM_177578
  Red=Cloning site Green=Tags(s)
  
  MGSIQIIFAAYCVVLCVLQMLVLSSEQFTITGLERPVLAPLGGILELSCQLSPPQNAQQMEIRWFRNRYT
  EPVYLYRNGKDLHGETISKYVERTELLKHDIGKGKVTLRVFKVTVDDDGSYHCVFKDGIFYEEHITEVKV
  TATSSDIKIIMHPPNIKGVMLECHSRGWFPQPHMEWRDSNGQVIPATSKSQSQDENKLFNMTMNLFADVG
  LHQIVTCYIQNLLTHQEESISIVLTVPISDQHFELDTLYLEDISVILCVVIVFNLKLNLLTYYRLERKYD
  GCTPGCKACFYILKIIIIILPFVFTFGCYNAIFLKYHQLQKKVSIPDPLYYFYTSWLVNMEMLGVFLVFF
  PTFINLIEFSQFIKTVPKPIWLCQENMREDDAIRHR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177578
• ORF Size: 1158 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177578.4, NP_808246.1
• RefSeq Size: 3627 bp
• RefSeq ORF: 1161 bp
• Locus ID: 195564
• UniProt ID: A7TZF0
• Cytogenetics: 4 D1
• Gene Summary: May act by engaging a cell surface molecule on immature T-cells in the embryonic thymus.[UniProtKB/Swiss-Prot Function]