Necab2 (NM_054095) Mouse Untagged Clone

CAT#: MC212297

Necab2 (untagged) - Mouse N-terminal EF-hand calcium binding protein 2 (Necab2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Necab2
• Synonyms: Efcbp2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212297 representing NM_054095
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCGAGCGGGCGGCGCGCCTGTGCAGGGCCGGCGCGCACAGGCTGCTCCGGGAGCCGCCGCCGCAGG
  GCCGCGCGCTGGGCGGGCTGCTGCGCTGGGTGGGCGCCAGGATGGGGGAACCCCGTGCACCGCTCGTCCC
  CGACATCCCCTCCGCGGACCCCGGCCCCGGGCCCGCCGCATCGCGAGGGGGCACAGCCGTCATCCTGGAC
  ATTTTCCGCCGCGCCGATAAAAATGATGATGGGAAGCTGTCCCTGGAGGAGTTTCAACTCTTCTTCGCAG
  ATGGCGTCCTTAATGAGAAGGAGCTGGAGGGCCTCTTCCATACCATCGATTCAGACAACACCAACCACGT
  GGACACCAAGGAACTGTGTGACTACTTTGTGGAGCACATGGGAGACTATGAAGATGTCCTGGCCTCCCTG
  GAGACTTTGAACCACTCAGTCCTGAAGGCCATGGGCTACACTAAGAAGGTGTATGAAGGTGGGAGCAATG
  TGGACCAGTTTGTGACACGCTTCCTTCTGAAGGAGACAGCCAATCAGATCCAGTCGCTGCTCAGCTCTGT
  GGAAAGTGCTGTAGAAGCCATCGAAGAACAGACCAGCCAGATCCGACAGGACCACTGCAAACCCAGCCAT
  GCCGTCAACGAGAGCCGCTACGGAGGCCCTACTCCTCCCTACATCCCCAACCACAAGCTTGTGGCCCCGG
  AGCCGATGAAGAGCCTGCCTGTGGCCACAGGGGAGCCAAAGGAGGATGGGCTGGAGGGCCAGATAAGCCG
  GTTGGCAGAGCTGATTGGGAGACTGGAGAGCAAGACCCTCTCGTTTGACCTGCAACAGCGCCTCTCGGAT
  GAAGAAGGCACCAACATGCACCTGCAGCTGGTCCGGCAGGAAATGGCCGTGTGCCCTGAGCAGCTGAGTG
  AGTTCTTGGATTCCCTGAGACAGTACCTCCGGAGCACGGCTGAAGAAAGGAACTGCTTCCATGTTGCCGC
  AGTGAGGATGGCAGATGGCCTAACCTTCGTCATCTACGAGTTCTGGGAGACAGAGGAGGAGTGGAAGAGG
  CACCTGCAGAGCCCTGTGTGCAAGGCCTTCCGACACGTCAAGGTTGACACGCTGAGCCAGCCTGAAGCAC
  TCTCCCAGATCTCCGTGCCAGCTGCCTGGTGCACCTCTGGCCGAGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_054095
• Insert Size: 1170 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_054095.2, NP_473436.1
• RefSeq Size: 1971 bp
• RefSeq ORF: 1170 bp
• Locus ID: 117148
• UniProt ID: Q91ZP9
• Cytogenetics: 8 E1
• Gene Summary: May act as a signaling scaffold protein that senses intracellular calcium. Can modulate ligand-induced internalization of ADORA2A and coupling efficiency of mGluR5/GRM5; for both receptors may regulate signaling activity such as promoting MAPK1/3 (ERK1/2) activation.[UniProtKB/Swiss-Prot Function]