U2af1 (NM_001163769) Mouse Untagged Clone

CAT#: MC212173

U2af1 (untagged) - Mouse U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 (U2af1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: U2af1
• Synonyms: 35kDa; 2010107D16Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212173 representing NM_001163769
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAATACTTGGCCTCCATCTTCGGCACCGAAAAAGACAAAGTCAACTGTTCATTTTATTTCAAAA
  TCGGAGCATGTCGTCATGGAGACAGATGTTCTCGGTTGCACAATAAACCAACCTTTAGCCAGACCATCTT
  GATTCAAAACATCTATCGTAATCCCCAAAACAGTGCACAGACGGCTGACGGCTCACACTGTGCTGTGAGC
  GACGTGGAGATGCAGGAGCACTATGATGAGTTCTTTGAGGAAGTCTTCACTGAGATGGAAGAGAAGTACG
  GGGAAGTCGAGGAGATGAACGTCTGCGACAACCTAGGGGACCACCTGGTGGGGAACGTGTATGTCAAGTT
  TCGACGTGAGGAGGATGCAGAGAAAGCCGTGATCGACTTGAATAACCGTTGGTTTAATGGACAGCCGATC
  CATGCAGAGCTGTCCCCAGTAACTGACTTCAGGGAAGCCTGCTGCCGCCAGTATGAAATGGGAGAGTGCA
  CAAGAGGGGGCTTCTGCAACTTCATGCATTTGAAGCCCATCTCAAGAGAGCTACGACGGGAGCTGTATGG
  GCGCCGGCGCAAGAAGCATAGATCCAGGTCCCGATCCAGGGAACGGCGTTCCCGATCCAGAGACCGTGGA
  CGCGGTGGTGGAGGTGGAGGTGGAGGTGGCGGAGGACGGGAGCGTGACAGGAGGCGGTCAAGAGACCGGG
  AGAGATCTGGACGATTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001163769
• Insert Size: 720 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163769.1, NP_001157241.1
• RefSeq Size: 907 bp
• RefSeq ORF: 720 bp
• Locus ID: 108121
• UniProt ID: Q9D883
• Cytogenetics: 17 B1
• Gene Summary: Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate coding exon but contains another compared to variant 1. Variants 1 and 2 are the same length, as are isoforms 1 and 2; however, their sequences vary slightly.