Klhdc8b (NM_030075) Mouse Untagged Clone

CAT#: MC211866

Klhdc8b (untagged) - Mouse kelch domain containing 8B (Klhdc8b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Klhdc8b
• Synonyms: 4931406O17Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211866 representing NM_030075
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCAGGAGGTGGTCGGGCCTTTGCTTGGCAGGTGTTCCCCCCAATGCCGACTTGCCGGGTATATG
  GTACAGTGGCACACCAGGACGGACACCTGCTGGTGTTGGGGGGCTGTGGCCGGGCTGGGTTACCTCTGGA
  TACTGCTGAGACTCTGGACATGGGCTCACACACATGGCTAGCACTAGCACCCCTGCCCACTGCCCGGGCT
  GGTGCCGCTGCTGTGGTTCTGGGTAAGCAGGTGCTAGTGGTAGGCGGCGTGGACGAAGTCCAAAGCCCAG
  TAGCTGCTGTGGAGGCCTTCTTGGCTGACGAAGGCCGCTGGGAGCGCCGGGCTACCCTCCCTCAAGCGGC
  CATGGGTGTTGCGACTGTGGAGAGAGATGGTATGGTGTATGCTCTGGGGGGAATGGGTCCTGACACGGCC
  CCCCAGGCCCAGGTACTGGTATATGAGCCCCGCCGGGACTGCTGGCTTTCGCTACCCTCCATGCCCACAC
  CCTGCTATGGGGCCTCCACCTTCCTGCACGGGAACAAGATCTATGTCTTGGGAGGCCGCCAGGGCAAGCT
  CCCAGTGACTGCTTTTGAAGCTTTTGATCTGGAGACCCGTACATGGACCCGACACCCAAGCCTGCCCAGT
  CGCAGAGCTTTTGCTGGCTGTGCTATGGCCGAAGGCAGTGTCTTTAGCCTGGGTGGCCTGCAGCAGCCTG
  GGCCCCACAATTTCTACTCCCGGCCGCACTTTGTCAACACTGTGGAGATGTTTGACCTGGAGCATGGCTC
  CTGGACTAAGCTGCCTCGTAGCCTGCGGATGAGGGATAAGAGGGCTGACTTTGTGGTTGGCTCCCTTGGG
  GGCAACATTGTGGCTATTGGGGGCCTTGGGAACCAGCCATGCCCTTTGGCCTCCGTGGAGAGCTTCAGTC
  TTGCACGGAGACGCTGGGAGGTGCTGCCTGCCATGCCTACGGCCCGATGCTCCTGCTCCAGCCTCCAGGC
  TGGGCCACGACTGTTTGTTATTGGGGGTGTGGCTCAGGGCCCAAGTCAAGCGGTGGAAGCACTGTGTCTA
  CGGGATGGAGTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_030075
• Insert Size: 1065 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030075.2, NP_084351.1
• RefSeq Size: 2953 bp
• RefSeq ORF: 1065 bp
• Locus ID: 78267
• UniProt ID: Q9D2D9
• Cytogenetics: 9 F2
• Gene Summary: Involved in pinching off the separated nuclei at the cleavage furrow and in cytokinesis. Required for mitotic integrity and maintenance of chromosomal stability. Protects cells against mitotic errors, centrosomal amplification, micronucleus formation and aneuploidy. Plays a key role of midbody function involving abscission of the daughter cells during cytokinesis and appropriate chromosomal and nuclear segregation into the daughter cells.[UniProtKB/Swiss-Prot Function]