Boll (NM_001113367) Mouse Untagged Clone

CAT#: MC211621

Boll (untagged) - Mouse bol, boule-like (Drosophila) (Boll), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Boll
• Synonyms: 4930554P13Rik; 4930597B14Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211621 representing NM_001113367
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCGGCTGGAACAATGTATCTGACCACTTCAACTGGGTACCCTTATACTTACCATAATGGCGTTG
  CTTATTTTCATACTCCAGAAGTAACTTCTGTCCCACCATCTTGGCCTTCACGTTCCATATCTAGTTCTCC
  TGTGATGGTGGCTCAGCCAGTTTATCAACAGCCTGCATATCACTATCAGGCCCCAGCACAGTGTTTACCA
  GGGCAGTGGCAGTGGGGTGTTCCCCAGTCCCCTGCTTCTTCTGCTCCATTCCTATATCTGCAACCTTCTG
  AGGTTATTTATCAGCCAGTAGAAATTGCGCAAGACGGTGGCTGTGTTCCTCCTCCACTGTCTCTGATGGA
  AACTTCTGTTCCAGAGCCTTATTCTGACCATGGAGTTCAAGCAGCATATCACCAGGTTTATGCTTCAAGT
  GCCATTGCTATGCCTGCACCTATGATGCAGCCTGAGCCAATTAAAGTAAGGAGTTTATTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001113367
• Insert Size: 483 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113367.1, NP_001106838.1
• RefSeq Size: 1087 bp
• RefSeq ORF: 483 bp
• Locus ID: 75388
• UniProt ID: Q924M5
• Cytogenetics: 1 C1.2
• Gene Summary: Probable RNA-binding protein, which may be required during spermatogenesis. May act by binding to the 3' UTR of mRNAs and regulating their translation (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses a different segment for its 5' end, compared to variant 1. This causes translation initiation at a downstream AUG and a protein (isoform 2) with a shorter N-terminus compared to isoform 1.